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This report describes a global marker of definitive endoderm, Claudin\u20106 (Cldn6). We report its expression in early development with particular attention to definitive endoderm derivatives. To create a genetic system to drive gene expression throughout the definitive endoderm with both spatial and temporal control, we target the endogenous locus with an inducible Cre recombinase (Cre\u2010ER<jats:sup>T2<\/jats:sup>) cassette. <jats:italic>Cldn6<\/jats:italic> null mice are viable and fertile with no obvious phenotypic abnormalities. We also report a lineage analysis of the fate of <jats:italic>Cldn6<\/jats:italic>\u2010expressing embryonic cells, which is relevant to the development of the pancreas, lung, and liver. Developmental Dynamics 237:504\u2013512, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.21437","type":"journal-article","created":{"date-parts":[[2008,1,22]],"date-time":"2008-01-22T02:16:23Z","timestamp":1200968183000},"page":"504-512","source":"Crossref","is-referenced-by-count":59,"title":["Genetic targeting of the endoderm with claudin\u20106<sup>CreER<\/sup>"],"prefix":"10.1002","volume":"237","author":[{"given":"William J.","family":"Anderson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Alcalde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu F.","family":"Kaneko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leah J.","family":"Blank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard I.","family":"Sherwood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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