{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:36:38Z","timestamp":1791268598602,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,7,15]],"date-time":"2008-07-15T00:00:00Z","timestamp":1216080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The pineal complex of zebrafish consists of a pineal organ and a left\u2010sided parapineal organ. Mutation of the <jats:italic>floating head<\/jats:italic> (<jats:italic>flh<\/jats:italic>) gene, which encodes a homeodomain protein, causes premature termination of pineal cell division without affecting specification or asymmetric placement of the parapineal. The <jats:italic>from beyond<\/jats:italic> (<jats:italic>fby<\/jats:italic>) mutation, a premature stop codon in the T\u2010domain\u2010containing protein Tbx2b, disrupts formation of the parapineal while leaving the pineal largely intact. However, <jats:italic>flh<\/jats:italic> is reported as being required for <jats:italic>tbx2b<\/jats:italic> transcription. To resolve the paradox that <jats:italic>flh<\/jats:italic>and <jats:italic>tbx2b<\/jats:italic> mutants have opposite phenotypes but have been placed in the same genetic pathway, we have examined transcriptional cross\u2010regulation in single <jats:italic>flh<\/jats:italic> or <jats:italic>fby<\/jats:italic> mutants and genetic epistasis in double mutants. Careful analysis shows that <jats:italic>flh<\/jats:italic> is not required for <jats:italic>tbx2b<\/jats:italic> transcription and double mutants exhibit an additive phenotype. We conclude that Flh and Tbx2b regulate separate programs of pineal and parapineal development. Developmental Dynamics 237:3538\u20133544, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.21607","type":"journal-article","created":{"date-parts":[[2008,7,15]],"date-time":"2008-07-15T13:42:28Z","timestamp":1216129348000},"page":"3538-3544","source":"Crossref","is-referenced-by-count":22,"title":["Formation of the asymmetric pineal complex in zebrafish requires two independently acting transcription factors"],"prefix":"10.1002","volume":"237","author":[{"given":"Corey D.","family":"Snelson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarred T.","family":"Burkart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua T.","family":"Gamse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,7,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00452"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.2001.19910063.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(03)00437-9"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.12.2703"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-133X(03)00148-0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng793"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00270"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00683-9"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"4257","DOI":"10.1242\/dev.121.12.4257","article-title":"Cell\u2010autonomous shift from axial to paraxial mesodermal development in zebrafish floating head mutants","volume":"121","author":"Halpern ME","year":"1995","journal-title":"Development"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1050050307"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1242\/dev.109.3.567","article-title":"Early onset of phenotype and cell patterning in the embryonic zebrafish retina","volume":"109","author":"Larison KD","year":"1990","journal-title":"Development"},{"key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"5101","DOI":"10.1242\/dev.127.23.5101","article-title":"Asymmetric nodal signaling in the zebrafish diencephalon positions the pineal organ","volume":"127","author":"Liang JO","year":"2000","journal-title":"Development"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(01)80045-3"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004270050158"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004270050014"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.016576"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0149-7634(82)90003-3"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/378150a0"},{"key":"e_1_2_6_20_1","first-page":"43","volume-title":"Methods in Cell Biology","author":"Walker C","year":"1999"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.21607","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.21607","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T15:28:29Z","timestamp":1760369309000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.21607"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7,15]]},"references-count":19,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["10.1002\/dvdy.21607"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.21607","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,7,15]]}}}