{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T04:10:37Z","timestamp":1760415037149,"version":"build-2065373602"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,11,4]],"date-time":"2008-11-04T00:00:00Z","timestamp":1225756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Embryonic day 13 mouse submandibular gland (E13\u2010SMG) rudiments with two to four clefts have been commonly used in culture experiments to show that growth factors, such as epidermal growth factor (EGF) \u2010family and fibroblast growth factor (FGF) \u2010family ligands, are involved in branching morphogenesis. In the present study, we focused on E12 rudiments and attempted to elucidate the roles of EGF\u2010 and FGF\u2010family ligands in SMG development from E12 to E13. In mesenchyme\u2010free, Matrigel\u2010embedded cultures, EGF + lysophosphatidic acid (LPA) induced branching in E13 epithelium, whereas E12 epithelium remained spherical and no branching occurred under the same culture conditions; however, both E12 and E13 epithelia elongated in response to FGF10. Reverse transcriptase\u2010polymerase chain reaction studies showed that the expression of <jats:italic>ErbB1<\/jats:italic> among four EGF receptors and <jats:italic>Lpa3<\/jats:italic> among three LPA receptors was lower in E12 than in E13 epithelia. <jats:italic>Fgf10<\/jats:italic>, <jats:italic>Fgf7<\/jats:italic>, and their major receptor <jats:italic>Fgfr2b<\/jats:italic> were highly and equally expressed in E12 and E13 rudiments. After 24 hr of mesenchyme\u2010free culture with FGF10 or FGF7, E12 epithelium was primed to initiate branching morphogenesis in response to EGF + LPA coincident with <jats:italic>ErbB1<\/jats:italic> and <jats:italic>Lpa3<\/jats:italic> up\u2010regulation. These results suggest that the EGF\u2010family ligand\u2013receptor system is undeveloped at E12 and that it becomes primed on E13 by the FGF ligand\u2013receptor system to play an important role in the induction of branching morphogenesis. Developmental Dynamics 238:315\u2013323, 2009.  \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.21780","type":"journal-article","created":{"date-parts":[[2008,11,4]],"date-time":"2008-11-04T16:39:04Z","timestamp":1225816744000},"page":"315-323","source":"Crossref","is-referenced-by-count":20,"title":["FGF alters epithelial competence for EGF at the initiation of branching morphogenesis of mouse submandibular gland"],"prefix":"10.1002","volume":"238","author":[{"given":"Mari","family":"Nitta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiaki","family":"Kume","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Nogawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,22]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.23.4867"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.63.1.471"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.10161"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.3.483"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215549704501208"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00172"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(19991101)256:3<252::AID-AR5>3.0.CO;2-6"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-5-11"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1447-073x.2005.00102.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199702)208:2<149::AID-AJA2>3.0.CO;2-I"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9639"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10309"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.2008.01053.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20078"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199906)215:2<148::AID-DVDY7>3.0.CO;2-V"},{"key":"e_1_2_5_17_1","first-page":"221","article-title":"Determination of the curvature of epithelial cell mass by mesenchyme in branching morphogenesis of mouse salivary gland","volume":"73","author":"Nogawa H","year":"1983","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.112.3.855"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20651"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3721"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.2006.00088.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.011171"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M709995200"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-1878(199801)20:1<41::AID-BIES7>3.0.CO;2-V"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01712"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1105396"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01690"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2007.01.006"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0351"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03505"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.21780","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.21780","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T03:56:33Z","timestamp":1760414193000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.21780"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1,22]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,2]]}},"alternative-id":["10.1002\/dvdy.21780"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.21780","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"type":"print","value":"1058-8388"},{"type":"electronic","value":"1097-0177"}],"subject":[],"published":{"date-parts":[[2009,1,22]]}}}