{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T04:28:14Z","timestamp":1767846494401,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,11,25]],"date-time":"2008-11-25T00:00:00Z","timestamp":1227571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Susceptibility to the rectal pathogen <jats:italic>Microbacterium nematophilum<\/jats:italic> provides a means of examining hindgut differentiation in <jats:italic>C. elegans<\/jats:italic>. Mutants of <jats:italic>bus<\/jats:italic>\u2010<jats:italic>1<\/jats:italic> are resistant to infection with this pathogen. We show here that <jats:italic>bus<\/jats:italic>\u2010<jats:italic>1<\/jats:italic> encodes a predicted acyltransferase expressed in rectal epithelial cells (K, F, and U), suggesting its involvement in regional surface modification. <jats:italic>bus<\/jats:italic>\u2010<jats:italic>1<\/jats:italic> reporter genes were used to show spatial regulation by hindgut developmental control genes: <jats:italic>egl<\/jats:italic>\u2010<jats:italic>38, mab<\/jats:italic>\u2010<jats:italic>9<\/jats:italic>, and <jats:italic>mab<\/jats:italic>\u2010<jats:italic>23<\/jats:italic>. A <jats:italic>bus<\/jats:italic>\u2010<jats:italic>1::GFP<\/jats:italic> reporter reveals the conspicuous rectal epithelial swelling induced by <jats:italic>M. nematophilum<\/jats:italic>. The <jats:italic>C. briggsae<\/jats:italic> ortholog of <jats:italic>bus<\/jats:italic>\u2010<jats:italic>1<\/jats:italic> exhibits conserved function and rectal expression, but it is expressed in vulval as well as rectal cells, correlated with pathogen adhesion to both vulval and rectal cells in this species. Another acyltransferase affecting bacterial adhesion, <jats:italic>bus<\/jats:italic>\u2010<jats:italic>18<\/jats:italic>\/<jats:italic>acl<\/jats:italic>\u2010<jats:italic>10<\/jats:italic>, was also identified, which also shows strong rectal expression, but it is expressed in additional epithelial tissues and is required for general surface integrity. Developmental Dynamics 237:3762\u20133776, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.21792","type":"journal-article","created":{"date-parts":[[2008,11,25]],"date-time":"2008-11-25T16:46:27Z","timestamp":1227631587000},"page":"3762-3776","source":"Crossref","is-referenced-by-count":17,"title":["The acyltransferase gene <i>bus\u20101<\/i> exhibits conserved and specific expression in nematode rectal cells and reveals pathogen\u2010induced cell swelling"],"prefix":"10.1002","volume":"237","author":[{"given":"Maria 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