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Here, we show that the founding member of this subfamily, <jats:italic>Brd2<\/jats:italic>, is an essential gene by generating a mutant mouse line lacking Brd2 function. Homozygous <jats:italic>Brd2<\/jats:italic> mutants are embryonic lethal, with most <jats:italic>Brd2<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> embryos dying by embryonic day 11.5. Before death, the homozygous embryos were notably smaller and exhibited abnormalities in the neural tube where the gene is highly expressed. Brd2\u2010deficient embryonic fibroblast cells were observed to proliferate more slowly than controls. Experiments to explore whether placental insufficiency could be a cause of the embryonic lethality showed that injecting diploid mutant embryonic stem cells into tetraploid wild\u2010type blastocysts did not rescue the lethality; that is Brd2\u2010deficient embryos could not be rescued by wild\u2010type extraembryonic tissues. Furthermore, there were enhanced levels of cell death in Brd2\u2010deficient embryos. Developmental Dynamics 238:908\u2013917, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.21911","type":"journal-article","created":{"date-parts":[[2009,3,19]],"date-time":"2009-03-19T12:22:32Z","timestamp":1237465352000},"page":"908-917","source":"Crossref","is-referenced-by-count":151,"title":["Double bromodomain\u2010containing gene <i>Brd2<\/i> is essential for embryonic development in mouse"],"prefix":"10.1002","volume":"238","author":[{"given":"Enyuan","family":"Shang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duancheng","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Greenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debra J.","family":"Wolgemuth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,3,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M407435200"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0733-8619(18)30108-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00414-2"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/10425179209020804"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2001.02355.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2005-0444"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1167.2007.00977.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00692-07"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devbrainres.2003.12.011"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.3.261"},{"key":"e_1_2_6_12_1","first-page":"417","article-title":"RING3 kinase transactivates promoters of cell cycle regulatory genes through E2F","volume":"11","author":"Denis GV","year":"2000","journal-title":"Cell Growth Differ"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr050430u"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-8-39"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(86)90392-1"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000066748"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.41.10.1651"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.2741\/Florence"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21432"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/150.4.1497"},{"key":"e_1_2_6_21_1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1093\/genetics\/81.4.683","article-title":"Isolation and characterization of sex\u2010linked female\u2010sterile mutants in Drosophila melanogaster","volume":"81","author":"Gans M","year":"1975","journal-title":"Genetics"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1093\/genetics\/96.4.887","article-title":"The role of dosage of the region 7D1\u20137D5\u20136 of the X chromosome in the production of homeotic transformations in Drosophila melanogaster","volume":"96","author":"Gans M","year":"1980","journal-title":"Genetics"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1320310125"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1086\/302763"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-06-2116"},{"key":"e_1_2_6_26_1","doi-asserted-by":"crossref","first-page":"3085","DOI":"10.1242\/jcs.113.17.3085","article-title":"Activation\u2010induced nuclear translocation of RING3","volume":"113","author":"Guo N","year":"2000","journal-title":"J Cell Sci"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(89)90094-8"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/20.10.2603"},{"key":"e_1_2_6_29_1","first-page":"497","volume-title":"Manipulating the mouse embryo. 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