{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T21:01:27Z","timestamp":1790974887331,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,5,4]],"date-time":"2009-05-04T00:00:00Z","timestamp":1241395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mutations in human TBX22 cause X\u2010linked cleft palate with ankyloglossia syndrome (CPX; OMIM 303400). Since the secondary palate was an adaptation to breathing on land, we characterized zebrafish <jats:italic>tbx22<\/jats:italic> to study molecular mechanisms regulating early vertebrate craniofacial patterning. Rapid Amplification of cDNA Ends (RACE) analyses revealed two zebrafish <jats:italic>tbx22<\/jats:italic> splice isoforms, <jats:italic>tbx22\u20101<\/jats:italic> and <jats:italic>tbx22\u20102<\/jats:italic>, encoding proteins of 444 and 400 amino acids, respectively. <jats:italic>tbx22\u20101<\/jats:italic> resembles canonical Tbx22 orthologs, while <jats:italic>tbx22\u20102<\/jats:italic> lacks conserved N\u2010terminal sequence. Developmental RT\u2010PCR revealed that <jats:italic>tbx22\u20101<\/jats:italic> is maternally and zygotically expressed, while <jats:italic>tbx22\u20102<\/jats:italic> is expressed zygotically. WISH analyses revealed strong <jats:italic>tbx22<\/jats:italic> mRNA expression in ectomesenchyme underlying the stomodeum, a bilaminar epithelial structure demarcating early mouth formation, and in early presumptive jaw joints. Zebrafish <jats:italic>tbx22<\/jats:italic> expression mirrored some aspects of mammalian Tbx22, consistent with roles in early vertebrate face patterning. These studies identify an early transcription factor governing vertebrate facial development, which may underlie common craniofacial birth disorders. Developmental Dynamics 238:1605\u20131612, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.21962","type":"journal-article","created":{"date-parts":[[2009,5,4]],"date-time":"2009-05-04T21:06:35Z","timestamp":1241471195000},"page":"1605-1612","source":"Crossref","is-referenced-by-count":12,"title":["Alternative splicing, phylogenetic analysis, and craniofacial expression of zebrafish <i>tbx22<\/i>"],"prefix":"10.1002","volume":"238","author":[{"given":"P. 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