{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:15:06Z","timestamp":1790871306552,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,9,23]],"date-time":"2009-09-23T00:00:00Z","timestamp":1253664000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Mrj co\u2010chaperone is expressed throughout the mouse conceptus, yet its requirement for placental development has prohibited a full understanding of its embryonic function. Here, we show that <jats:italic>Mrj<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> embryos exhibit neural tube defects independent of the placenta phenotype, including exencephaly and thin\u2010walled neural tubes. Molecular analyses revealed fewer proliferating cells and a down\u2010regulation of early neural progenitor (<jats:italic>Pax6<\/jats:italic>, <jats:italic>Olig2<\/jats:italic>, <jats:italic>Hes5<\/jats:italic>) and neuronal (<jats:italic>Nscl2<\/jats:italic>, <jats:italic>SCG10<\/jats:italic>) cell markers in <jats:italic>Mrj<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> neuroepithelial cells. Furthermore, <jats:italic>Mrj<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> neurospheres are significantly smaller and form fewer secondary neurospheres indicating that Mrj is necessary for self\u2010renewal of neural stem cells. However, the molecular function of Mrj in this context remains elusive because Mrj does not colocalize with Bmi\u20101, a self\u2010renewal protein. Furthermore, unlike in <jats:italic>Mrj<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> placentas, intermediate filament\u2010containing aggregates do not accumulate in <jats:italic>Mrj<jats:sup>\u2212\/\u2212<\/jats:sup><\/jats:italic> neuroepithelium, ruling out nestin as a substrate for Mrj. Regardless, Mrj plays an important role in neural stem cell self\u2010renewal. Developmental Dynamics 238:2564\u20132574, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.22088","type":"journal-article","created":{"date-parts":[[2009,9,23]],"date-time":"2009-09-23T15:23:49Z","timestamp":1253719429000},"page":"2564-2574","source":"Crossref","is-referenced-by-count":28,"title":["Neural stem cell self\u2010renewal requires the Mrj co\u2010chaperone"],"prefix":"10.1002","volume":"238","author":[{"given":"Erica D.","family":"Watson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Mattar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol","family":"Schuurmans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James 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