{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:57:12Z","timestamp":1760403432262,"version":"build-2065373602"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T00:00:00Z","timestamp":1597017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hedgehog signaling has various regulatory functions in tissue morphogenesis and differentiation. To investigate its involvement in anterior pituitary precursor development and the lens precursor potential for anterior pituitary precursors, we investigated <jats:italic>Talpid<\/jats:italic> mutant Japanese quail embryos, in which hedgehog signaling is defective.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Talpid<\/jats:italic> mutants develop multiple pituitary precursor\u2010like pouches of variable sizes from the oral ectoderm (OE). The ectopic pituitary pouches initially express the pituitary\u2010associated transcription factor (TF) LHX3 similarly to Rathke's pouch, the genuine pituitary precursor. The pouches coexpress the TFs SOX2 and PAX6, a signature of lens developmental potential. Most <jats:italic>Talpid<\/jats:italic> mutant pituitary pouches downregulate LHX3 expression and activate the lens\u2010essential TF PROX1, leading to the development of small lens tissue expressing \u03b1\u2010, \u03b2\u2010, and \u03b4\u2010crystallins. In contrast, mutant Rathke's pouches express a lower level of LHX3, which is primarily localized in the cytoplasm, and activate the lens developmental pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Hedgehog signaling in normal embryos regulates the development of Rathke's pouch in two steps. First, by confining Rathke's pouch development in a low hedgehog signaling region of the OE. Second, by sustaining LHX3 activity to promote anterior pituitary development, while inhibiting ectopic lens development.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.222","type":"journal-article","created":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T07:23:46Z","timestamp":1594106626000},"page":"1425-1439","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The formation of multiple pituitary pouches from the oral ectoderm causes ectopic lens development in hedgehog signaling\u2010defective avian embryos"],"prefix":"10.1002","volume":"249","author":[{"given":"Yuki","family":"Taira","sequence":"first","affiliation":[{"name":"Faculty of Life Sciences Kyoto Sangyo University  Kyoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuya","family":"Ikuta","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences Kyoto Sangyo University  Kyoto 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