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The mechanism by which temperature is transduced into a sex\u2010determining molecular signal remains a mystery, and here we examine the molecular network underlying sex determination in gonads in vitro. We use a whole organ culture system to show that expression of putative members of the sex\u2010determining network (<jats:italic>Dmrt1<\/jats:italic>, <jats:italic>Sox9<\/jats:italic>, <jats:italic>Mis<\/jats:italic>, and <jats:italic>FoxL2<\/jats:italic>) are regulated by temperature endogenously within cells in the bipotential gonad and do not require other embryonic tissues to be expressed in a normal pattern in the red\u2010eared slider turtle, <jats:italic>Trachemys scripta<\/jats:italic>. Furthermore, following a change in temperature, these factors exhibit temperature\u2010responsive expression patterns that last for the duration of gonadogenesis. Finally, mosaic misexpression of a fusion <jats:italic>Sox9<\/jats:italic> construct demonstrates the ability to functionally manipulate the gonad at the molecular level. Developmental Dynamics 239:1061\u20131075, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.22266","type":"journal-article","created":{"date-parts":[[2010,3,16]],"date-time":"2010-03-16T15:12:01Z","timestamp":1268752321000},"page":"1061-1075","source":"Crossref","is-referenced-by-count":67,"title":["Genetic network underlying temperature\u2010dependent sex determination is endogenously regulated by temperature in isolated cultured <i>Trachemys scripta<\/i> gonads"],"prefix":"10.1002","volume":"239","author":[{"given":"Christina 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