{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T15:54:29Z","timestamp":1777650869513,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,4,20]],"date-time":"2010-04-20T00:00:00Z","timestamp":1271721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cranial neural crest cells (NCCs) require neuropilin signaling to reach and invade the branchial arches. Here, we use an in vivo chick model to investigate whether the neuropilin\u20101 knockdown phenotype is specific to the second branchial arch (ba2), changes in NCC behaviors and phenotypic consequences, and whether neuropilins work together to facilitate entry into and invasion of ba2. We find that cranial NCCs with reduced neuropilin\u20101 expression displayed shorter protrusions and decreased cell body and nuclear length\u2010to\u2010width ratios characteristic of a loss in polarity and motility, after specific interaction with ba2. Directed NCC migration was rescued by transplantation of transfected NCCs into rhombomere 4 of younger hosts. Lastly, reduction of neuropilin\u20102 expression by shRNA either solely or with reduction of neuropilin\u20101 expression did not lead to a stronger head phenotype. Thus, NCCs, independent of rhombomere origin, require neuropilin\u20101, but not neuropilin\u20102 to maintain polarity and directed migration into ba2. Developmental Dynamics 239:1664\u20131673, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.22303","type":"journal-article","created":{"date-parts":[[2010,4,20]],"date-time":"2010-04-20T15:30:22Z","timestamp":1271777422000},"page":"1664-1673","source":"Crossref","is-referenced-by-count":21,"title":["Neuropilin\u20101 interacts with the second branchial arch microenvironment to mediate chick neural crest cell dynamics"],"prefix":"10.1002","volume":"239","author":[{"given":"Rebecca","family":"McLennan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul 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