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Individual epithelial cells migrated dynamically but erratically. The epithelial cleft extended by wiggling and separated a cluster of cells into two buds during branching. We examined the ultrastructure of the clefts in SMG rudiments treated with the laminin peptide A5G77f, which induces epithelial clefting. A short cytoplasmic shelf with a core of microfilaments was found at the deep end of the cleft. We propose that epithelial clefting involves a dynamic movement of cells at the base of the cleft, and the formation of a shelf within a cleft cell. The shelf might form a matrix attachment point at the base of the cleft with a core of microfilaments driving cleft elongation. Developmental Dynamics 239:1739\u20131747, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.22312","type":"journal-article","created":{"date-parts":[[2010,5,12]],"date-time":"2010-05-12T12:39:04Z","timestamp":1273667944000},"page":"1739-1747","source":"Crossref","is-referenced-by-count":26,"title":["Cellular dynamics of epithelial clefting during branching morphogenesis of the mouse submandibular gland"],"prefix":"10.1002","volume":"239","author":[{"given":"Yuichi","family":"Kadoya","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shohei","family":"Yamashina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,5,17]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"1131","volume-title":"Molecular biology of the cell","author":"Alberts 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