{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T03:30:30Z","timestamp":1768015830258,"version":"3.49.0"},"reference-count":69,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2010,7,22]],"date-time":"2010-07-22T00:00:00Z","timestamp":1279756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The specific roles of classical cadherins at key morphogenetic events during development are still not fully understood. As part of a project to study cadherin function during early mammalian development, we generated mice carrying an HA\u2010epitope tagged <jats:italic>Cdh1<\/jats:italic> (E\u2010cadherin) cDNA knocked into the <jats:italic>Cdh1<\/jats:italic> locus, similar to the previously described mouse mutants in which we forced <jats:italic>Cdh2<\/jats:italic> (N\u2010cadherin) expression in the <jats:italic>Cdh1<\/jats:italic> expression domain. As expected and in contrast to <jats:italic>Cdh1<\/jats:italic><jats:sup><jats:italic>Cdh2\/Cdh2<\/jats:italic><\/jats:sup> and <jats:italic>Cdh1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>, our <jats:italic>Cdh1<\/jats:italic><jats:sup><jats:italic>HA\/HA<\/jats:italic><\/jats:sup> mutant embryos form proper trophectoderm, implant and undergo both gastrulation and neurulation. However, <jats:italic>Cdh1<\/jats:italic><jats:sup><jats:italic>HA\/HA<\/jats:italic><\/jats:sup> mice display an unexpected phenotype at embryonic day 10.5. <jats:italic>Cdh1<\/jats:italic><jats:sup><jats:italic>HA\/HA<\/jats:italic><\/jats:sup> embryos are smaller, paler and suffer from an insufficient nutrient supply. We detected a reduced expression of <jats:italic>Cdh1<\/jats:italic><jats:sup><jats:italic>HA<\/jats:italic><\/jats:sup> specifically in the extraembryonic ectoderm and in the labyrinth layer, whereas expression in the embryo proper was normal. With this approach, we show for the first time that Cdh1 is essential for the correct formation of the placenta. Placentas without <jats:italic>Cdh1<\/jats:italic> expression are impaired and incapable of establishing a proper connection between the embryonic and the maternal blood vessels for efficient nutrient and oxygen transport. Developmental Dynamics 239:2330\u20132344, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.22375","type":"journal-article","created":{"date-parts":[[2010,7,22]],"date-time":"2010-07-22T09:59:12Z","timestamp":1279792752000},"page":"2330-2344","source":"Crossref","is-referenced-by-count":25,"title":["A <i>Cdh1<sup>HA<\/sup><\/i> knock\u2010in allele rescues the <i>Cdh1<sup>\u2212\/\u2212<\/sup><\/i> phenotype but shows essential Cdh1 function during placentation"],"prefix":"10.1002","volume":"239","author":[{"given":"Marc P.","family":"Stemmler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan","family":"Bedzhov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,7,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0710504105"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(05)00014-6"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81618-9"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2006.09.004"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/376768a0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-006-0343-x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(02)00090-4"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-005-0051-7"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1242\/dev.113.3.767","article-title":"E\u2010cadherin expression during the differentiation of human trophoblasts","volume":"113","author":"Coutifaris C","year":"1991","journal-title":"Development"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.2006.00103.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01316"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.022707"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.20042"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/cac.10.2.105.118"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/6.10.943"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1530\/reprod\/119.1.59"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/371333a0"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1699"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(98)00093-8"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2393-9-8"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/320447a0"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M210081200"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.8.1839"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22075"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02595"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.20128"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.127.5.1375"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02722"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2009.284"},{"key":"e_1_2_6_31_1","first-page":"45","article-title":"Reactivity of monoclonal antibodies against intermediate filament proteins during embryonic development","volume":"64","author":"Kemler R","year":"1981","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.19.4179"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207831"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209091"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.17.8263"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(03)00052-5"},{"key":"e_1_2_6_37_1","first-page":"3819","article-title":"N\u2010cadherin\u2010mediated intercellular interactions promote survival and migration of melanoma cells","volume":"61","author":"Li G","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_6_38_1","first-page":"275","article-title":"Phenotypic analysis of the mouse placenta","volume":"121","author":"Natale DR","year":"2006","journal-title":"Methods Mol Med"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.072410mn"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(09)89002-9"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.6.2649"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1053\/plac.2002.0802"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006578200"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.27.19347"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.0148"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.4.1025"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.3.855"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/35080570"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/373699a0"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/23.24.5080"},{"key":"e_1_2_6_51_1","doi-asserted-by":"crossref","first-page":"706","DOI":"10.1053\/plac.2002.0864","article-title":"The role of E\u2010cadherin in the motility and invasion of implantation site intermediate trophoblast","volume":"23","author":"Shih Ie M","year":"2002","journal-title":"Placenta"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7618085"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.020099"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/5007"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1039\/b719215k"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10301"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01662"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.102.4.639"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.151.3.563"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-471-1_15"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1835"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600605"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-8465-8"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(84)90241-6"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00001.2005"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.4.753"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1438.2003.13400.x"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.8.3789"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119387"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.03.048"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.22375","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.22375","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T16:02:57Z","timestamp":1760371377000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.22375"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7,22]]},"references-count":69,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2010,9]]}},"alternative-id":["10.1002\/dvdy.22375"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.22375","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7,22]]}}}