{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:00:10Z","timestamp":1790485210637,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,11,18]],"date-time":"2010-11-18T00:00:00Z","timestamp":1290038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To aid in detection and tracking of cells targeted by the left\u2010right (LR) pathway in the heart throughout morphogenesis, expression from a <jats:italic>Pitx2c\u2010lacZ<\/jats:italic> transgene (<jats:italic>P2Ztg<\/jats:italic>) was analysed in detail. \u03b2\u2010galactosidase expression from <jats:italic>P2Ztg<\/jats:italic> was robust, allowing reliable visualisation of low\u2010level <jats:italic>Pitx2c<\/jats:italic> expression, and was virtually entirely dependent upon NODAL signalling in the heart. <jats:italic>P2Ztg<\/jats:italic> showed expression in trabecular and septal, as well as non\u2010trabecular, myocardium, and a strong expression bias in myocardium associated with individual endocardial cushions of the atrioventricular canal and outflow tract, which are essential for cardiac septation. Expression on the ventral surface of the outflow tract evolved to a specific stripe that could be used to track the future aorta during outflow tract spiralling and remodelling. Our data show that the <jats:italic>P2Ztg<\/jats:italic> transgene is a useful resource for detection of molecular disturbances in the LR cascade, as well as morphogenetic defects associated with other cardiac congenital disorders. Developmental Dynamics, 2011. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.22492","type":"journal-article","created":{"date-parts":[[2010,11,18]],"date-time":"2010-11-18T09:39:38Z","timestamp":1290073178000},"page":"195-203","source":"Crossref","is-referenced-by-count":39,"title":["Characterization of <i>Pitx2c<\/i> expression in the mouse heart using a reporter transgene"],"prefix":"10.1002","volume":"240","author":[{"given":"Milena B.","family":"Furtado","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Biben","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidetaka","family":"Shiratori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Hamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard P.","family":"Harvey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,11,18]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.06.009"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/heart.89.8.949"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.8.4573"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000202800.85341.6e"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1710"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.0133"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201737200"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000141429.13560.cb"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02693"},{"key":"e_1_2_5_11_1","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1242\/dev.127.5.1081","article-title":"Mesendoderm and left\u2010right brain, heart and gut development are differentially regulated by pitx2 isoforms","volume":"127","author":"Essner JJ","year":"2000","journal-title":"Development"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-1738(03)00039-2"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1682108"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.20.4643"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(200005)218:1<195::AID-DVDY17>3.0.CO;2-C"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)01084-X"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.24.5749"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/45803"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0700923104"},{"key":"e_1_2_5_20_1","doi-asserted-by":"crossref","first-page":"2039","DOI":"10.1242\/dev.128.11.2039","article-title":"Regulation of left\u2010right asymmetry by thresholds of Pitx2c activity","volume":"128","author":"Liu C","year":"2001","journal-title":"Development"},{"key":"e_1_2_5_21_1","doi-asserted-by":"crossref","first-page":"5081","DOI":"10.1242\/dev.129.21.5081","article-title":"Pitx2c patterns anterior myocardium and aortic arch vessels and is required for local cell movement into atrioventricular cushions","volume":"129","author":"Liu C","year":"2002","journal-title":"Development"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00849"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/381158a0"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.10.1831"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/45797"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0009-9163.2004.00258.x"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199705)248:1<84::AID-AR10>3.0.CO;2-1"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02309"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmg.2005.12.003"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.a.20269"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02292"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/29004"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.15.3437"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00227-0"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00162-9"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02470"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9547"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.2.329"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.163188"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199607)206:3<301::AID-AJA7>3.0.CO;2-M"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.22492","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.22492","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T09:37:50Z","timestamp":1760348270000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.22492"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11,18]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["10.1002\/dvdy.22492"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.22492","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11,18]]}}}