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We and others have recently mapped the dominant preaxial polydactyly (Po) locus in Silkie chickens to a single nucleotide polymorphism (SNP) in the ZRS region. Using polymorphisms in the chicken <jats:italic>Shh<\/jats:italic> sequence, we confirm that the ZRS region directly regulates <jats:italic>Shh<\/jats:italic> expression in the developing limb causing ectopic <jats:italic>Shh<\/jats:italic> expression in the anterior leg, prolonged <jats:italic>Shh<\/jats:italic> expression in the posterior limb, and allelic imbalance between wt and Slk <jats:italic>Shh<\/jats:italic> alleles in heterozygote limbs. Using Silkie legs, we have explored the consequences of increased <jats:italic>Shh<\/jats:italic> expression in the posterior leg on the patterning of the toes, and the induction of preaxial polydactyly. Developmental Dynamics 240:1163\u20131172, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.22623","type":"journal-article","created":{"date-parts":[[2011,4,4]],"date-time":"2011-04-04T12:33:32Z","timestamp":1301920412000},"page":"1163-1172","source":"Crossref","is-referenced-by-count":46,"title":["The chicken polydactyly (Po) locus causes allelic imbalance and ectopic expression of <i>Shh<\/i> during limb development"],"prefix":"10.1002","volume":"240","author":[{"given":"Ian C.","family":"Dunn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. 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