{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T12:01:28Z","timestamp":1775908888472,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,4,20]],"date-time":"2011-04-20T00:00:00Z","timestamp":1303257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The evolutionarily conserved, non\u2010coding \u223c800\u2010base\u2010pair (bp) zone of polarizing activity (ZPA) regulatory sequence (<jats:italic>ZRS<\/jats:italic>) controls <jats:italic>Shh<\/jats:italic> expression in the posterior limb. We report that the chicken mutant <jats:italic>oligozeugodactyly<\/jats:italic> (<jats:italic>ozd<\/jats:italic>), which lacks limb <jats:italic>Shh<\/jats:italic> expression, has a large deletion within the <jats:italic>ZRS<\/jats:italic>. Furthermore, the preaxial polydactylous, Silkie Breed chicken, which develops ectopic anterior limb <jats:italic>Shh<\/jats:italic> expression, has a single bp change within the <jats:italic>ZRS<\/jats:italic>. Using an in vivo reporter assay to examine enhancer function in the chick limb, we demonstrate that the wild\u2010type <jats:italic>ZRS<\/jats:italic> drives \u03b2\u2010galactosidase reporter expression in the ZPA of both wild\u2010type and <jats:italic>ozd<\/jats:italic> limbs. The Silkie ZRS drives \u03b2\u2010galactosidase in both posterior and anterior <jats:italic>Shh<\/jats:italic> domains in wild\u2010type limb buds. These results support the hypothesis that the <jats:italic>ZRS<\/jats:italic> integrates positive and negative prepatterned regulatory inputs in the chicken model system and demonstrate the utility of the chicken limb as an efficient genetic system for gene regulatory studies. Developmental Dynamics 240:1212\u20131222, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.22634","type":"journal-article","created":{"date-parts":[[2011,4,20]],"date-time":"2011-04-20T15:44:31Z","timestamp":1303314271000},"page":"1212-1222","source":"Crossref","is-referenced-by-count":32,"title":["Identification of spontaneous mutations within the long\u2010range limb\u2010specific <i>Sonic hedgehog<\/i> enhancer (<i>ZRS<\/i>) that alter <i>Sonic hedgehog<\/i> expression in the chicken limb mutants <i>oligozeugodactyly<\/i> and silkie breed"],"prefix":"10.1002","volume":"240","author":[{"given":"Sarah A.","family":"Maas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F.","family":"Fallon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,4,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2008.11.011"},{"key":"e_1_2_6_3_1","first-page":"969","article-title":"Unusual pattern of Sonic hedgehog expression in the polydactylous mouse mutant Hemimelic extra\u2010toes","volume":"46","author":"Blanc I","year":"2002","journal-title":"Int J Dev Biol"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.07.009"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"1971","DOI":"10.1242\/dev.121.7.1971","article-title":"Polydactylous limbs in Strong's Luxoid mice result from ectopic polarizing activity","volume":"121","author":"Chan DC","year":"1995","journal-title":"Development"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1242\/dev.127.11.2461","article-title":"The bHLH transcription factor dHAND controls Sonic hedgehog expression and establishment of the zone of polarizing activity during limb development","volume":"127","author":"Charite J","year":"2000","journal-title":"Development"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0346"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05436"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/jhered\/esp120"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1242\/dev.120.8.2213","article-title":"Cis\u2010acting regulatory sequences governing Wnt\u20101 expression in the developing mouse CNS","volume":"120","author":"Echelard Y","year":"1994","journal-title":"Development"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.2.281"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.10.2133"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1525"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702613200"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000901"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1050880104"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1086\/316955"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(00)00492-5"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.08.015"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00861"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10473"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.112212199"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg180"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddm370"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(98)00090-2"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(95)00156-4"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10502"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20254"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.02.005"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.13.1645"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1996.8457"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90626-2"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00245"},{"key":"e_1_2_6_34_1","doi-asserted-by":"crossref","first-page":"2319","DOI":"10.1242\/dev.122.8.2319","article-title":"The limb field mesoderm determines initial limb bud anteroposterior asymmetry and budding independent of sonic hedgehog or apical ectodermal gene expressions","volume":"122","author":"Ros MA","year":"1996","journal-title":"Development"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-066-7:245"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-033-2317-5"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01613"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.22.4811"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)80022-0"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.05.030"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1400930302"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.219202"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.024158"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(03)00088-1"},{"key":"e_1_2_6_45_1","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1093\/genetics\/29.3.217","article-title":"Inheritance of polydactylism in the fowl","volume":"29","author":"Warren DC","year":"1944","journal-title":"Genetics"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1096049"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2681"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.02.008"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.051789"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.22634","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.22634","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T09:35:07Z","timestamp":1760348107000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.22634"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,4,20]]},"references-count":48,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2011,5]]}},"alternative-id":["10.1002\/dvdy.22634"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.22634","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,4,20]]}}}