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Here, we report that ROCK\u2010mediated integrin activation and subsequent formation of focal adhesion complexes comprise this mechanochemical checkpoint. Inhibition of ROCK1 and nonmuscle myosin II activity decreased integrin \u03b21 activation in the cleft region and interfered with localization and activation of focal adhesion complex proteins, such as focal adhesion kinase (FAK). Inhibition of FAK activity also prevented cleft progression, by disrupting recruitment of the focal adhesion proteins talin and vinculin and subsequent fibronectin assembly in the cleft region while decreasing ERK1\/2 activation. These results demonstrate that inside\u2010out integrin signaling leading to a localized recruitment of active FAK\u2010containing focal adhesion protein complexes generates a mechanochemical checkpoint that facilitates progression of branching morphogenesis. Developmental Dynamics 240:2069\u20132083, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.22714","type":"journal-article","created":{"date-parts":[[2011,8,16]],"date-time":"2011-08-16T09:08:20Z","timestamp":1313485700000},"page":"2069-2083","source":"Crossref","is-referenced-by-count":44,"title":["A focal adhesion protein\u2010based mechanochemical checkpoint regulates cleft progression during branching morphogenesis"],"prefix":"10.1002","volume":"240","author":[{"given":"William P.","family":"Daley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua 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