{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T06:17:55Z","timestamp":1770963475558,"version":"3.50.1"},"reference-count":72,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,1,31]],"date-time":"2012-01-31T00:00:00Z","timestamp":1327968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:styled-content>Background<\/jats:styled-content>: Wiskott\u2010Aldrich Syndrome (WASP) family proteins participate in many cellular processes involving rearrangements of the actin cytoskeleton. To the date, four WASP subfamily members have been described in <jats:italic>Drosophila:<\/jats:italic> Wash, WASp, SCAR, and Whamy. Wash, WASp, and SCAR are essential during early <jats:italic>Drosophila<\/jats:italic> development where they function in orchestrating cytoplasmic events including membrane\u2010cytoskeleton interactions. A mutant for Whamy has not yet been reported. <jats:styled-content>Results<\/jats:styled-content>: We generated monoclonal antibodies that are specific to <jats:italic>Drosophila<\/jats:italic> Wash, WASp, SCAR, and Whamy, and use these to describe their spatial and temporal localization patterns. Consistent with the importance of WASP family proteins in flies, we find that Wash, WASp, SCAR, and Whamy are dynamically expressed throughout oogenesis and embryogenesis. For example, we find that Wash accumulates at the oocyte cortex. WASp is highly expressed in the PNS, while SCAR is the most abundantly expressed in the CNS. Whamy exhibits an asymmetric subcellular localization that overlaps with mitochondria and is highly expressed in muscle. <jats:styled-content>Conclusions<\/jats:styled-content>: All four WASP family members show specific expression patterns, some of which reflect their previously known roles and others revealing new potential functions. The monoclonal antibodies developed offer valuable new tools to investigate how WASP family proteins regulate actin cytoskeleton dynamics. Developmental Dynamics 241:608\u2013626, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.23742","type":"journal-article","created":{"date-parts":[[2012,1,13]],"date-time":"2012-01-13T11:54:42Z","timestamp":1326455682000},"page":"608-626","source":"Crossref","is-referenced-by-count":36,"title":["Developmental expression of <i>Drosophila<\/i> Wiskott\u2010Aldrich Syndrome family proteins"],"prefix":"10.1002","volume":"241","author":[{"given":"Evelyn","family":"Rodriguez\u2010Mesa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Teresa","family":"Abreu\u2010Blanco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicia E.","family":"Rosales\u2010Nieves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan 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