{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T18:15:28Z","timestamp":1774116928194,"version":"3.50.1"},"reference-count":83,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,3,23]],"date-time":"2012-03-23T00:00:00Z","timestamp":1332460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:styled-content>Background<\/jats:styled-content>: CCAAT\/enhancer\u2010binding protein (C\/EBP)\u03b1 is crucial for lung development and differentiation of the pulmonary epithelium. Conversely, no lung defects have been observed in C\/EBP\u03b2\u2010deficient mice, although C\/EBP\u03b2 trans\u2010activate pulmonary genes by binding to virtually identical DNA\u2010sequences as C\/EBP\u03b1. Thus, the pulmonary phenotype of mice lacking C\/EBP\u03b2 could be explained by functional replacement with C\/EBP\u03b1. We investigated whether C\/EBP\u03b1 and C\/EBP\u03b2 have overlapping functions in regulating lung epithelial differentiation during organogenesis. Epithelial differentiation was assessed in mice with a lung epithelial\u2013specific (<jats:italic>SFTPC<\/jats:italic>\u2010Cre\u2010mediated) deletion of C\/EBP\u03b1 (<jats:italic>Cebpa<\/jats:italic><jats:sup>\u0394LE<\/jats:sup>), C\/EBP\u03b2 (<jats:italic>Cebpb<\/jats:italic><jats:sup>\u0394LE<\/jats:sup>), or both genes (<jats:italic>Cebpa<\/jats:italic><jats:sup>\u0394LE<\/jats:sup>; <jats:italic>Cebpb<\/jats:italic><jats:sup>\u0394LE<\/jats:sup>). <jats:styled-content>Results<\/jats:styled-content>: Both <jats:italic>Cebpa<\/jats:italic><jats:sup>\u0394LE<\/jats:sup> mice and <jats:italic>Cebpa<\/jats:italic><jats:sup>\u0394LE<\/jats:sup>; <jats:italic>Cebpb<\/jats:italic><jats:sup>\u0394LE<\/jats:sup> mice demonstrated severe pulmonary immaturity compared to wild\u2010type littermates, while no differences in lung histology or epithelial differentiation were observed in <jats:italic>Cebpb<\/jats:italic><jats:sup>\u0394LE<\/jats:sup> mice. In contrast to <jats:italic>Cebpa<\/jats:italic><jats:sup>\u0394LE<\/jats:sup> mice, <jats:italic>Cebpa<\/jats:italic><jats:sup>\u0394LE<\/jats:sup>; <jats:italic>Cebpb<\/jats:italic><jats:sup>\u0394LE<\/jats:sup> mice also displayed undifferentiated Clara cells with markedly impaired protein and mRNA expression of Clara cell secretory protein (SCGB1A1), compared to wild\u2010type littermates. In addition, ectopic mucus\u2010producing cells were observed in the conducting airways of <jats:italic>Cebpa<\/jats:italic><jats:sup>\u0394LE<\/jats:sup>; <jats:italic>Cebpb<\/jats:italic><jats:sup>\u0394LE<\/jats:sup> mice. <jats:styled-content>Conclusions<\/jats:styled-content>: Our findings demonstrate that C\/EBP\u03b1 and C\/EBP\u03b2 play pivotal, and partly overlapping roles in determining airway epithelial differentiation, with possible implications for tissue regeneration in lung homeostasis and disease. Developmental Dynamics 241:911\u2013923, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.23773","type":"journal-article","created":{"date-parts":[[2012,3,12]],"date-time":"2012-03-12T14:17:31Z","timestamp":1331561851000},"page":"911-923","source":"Crossref","is-referenced-by-count":33,"title":["Airway epithelial cell differentiation during lung organogenesis requires C\/EBP\u03b1 and C\/EBP\u03b2"],"prefix":"10.1002","volume":"241","author":[{"given":"Abraham B.","family":"Roos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tove","family":"Berg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenny L.","family":"Barton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lukas","family":"Didon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magnus","family":"Nord","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,3,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.101.4.916"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.26.3.1109-1123.2006"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(76)80023-8"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00497.2005"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/jhg.2010.134"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.21.2.3691"},{"key":"e_1_2_6_8_1","first-page":"459","article-title":"Differential distribution of cytokeratins after microinjection of anti\u2010cytokeratin monoclonal antibodies","volume":"43","author":"Boller K","year":"1987","journal-title":"Eur J Cell Biol"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.26.3.4684"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1242\/jcs.108.2.811","article-title":"Tissue\u2010specific and efficient expression of the human simple epithelial keratin 8 gene in transgenic mice","volume":"108","author":"Casanova L","year":"1995","journal-title":"J Cell Sci"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00023.2003"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcbr.2000.0187"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.22.4.3916"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201293200"},{"key":"e_1_2_6_15_1","first-page":"2914","article-title":"SPDEF is required for mouse pulmonary goblet cell differentiation and regulates a network of genes associated with mucus production","volume":"119","author":"Chen G","year":"2009","journal-title":"J Clin Invest"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.19.7292-7299.2000"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.1.113-119.2000"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.127.4.1341"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00185008"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201007-1113OC"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2004-0060OC"},{"key":"e_1_2_6_22_1","first-page":"648","article-title":"Role of the Clara cell in renewal of the bronchiolar epithelium","volume":"38","author":"Evans MJ","year":"1978","journal-title":"Lab Invest"},{"key":"e_1_2_6_23_1","first-page":"126","article-title":"Role of nonciliated cells in renewal of the bronchial epithelium of rats exposed to NO2","volume":"123","author":"Evans MJ","year":"1986","journal-title":"Am J Pathol"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.17.3.2774"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.40.24753"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001850405"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64169-7"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.02.035"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-08-3181"},{"key":"e_1_2_6_30_1","first-page":"528","article-title":"Down\u2010regulation and antiproliferative role of C\/EBPalpha in lung cancer","volume":"62","author":"Halmos B","year":"2002","journal-title":"Cancer Res"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.12.7678"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.105.2.271"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009837"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.18.3913"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.117.5_suppl_1.251S"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.03.032"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.10068"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.17.8145"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.10.6014"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.07.021"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00071110"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02273"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00543-8"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.058669"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M305892200"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.7.4516"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V79.2.460.460"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2011.03.004"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1998.17.481"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb05527.x"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/jbiol3"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01678"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2011.100"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.7.3891"},{"key":"e_1_2_6_55_1","first-page":"71","article-title":"The cells of the tracheobronchial epithelium of the mouse: a quantitative light and electron microscope study","volume":"132","author":"Pack RJ","year":"1981","journal-title":"J Anat"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2005-0180OC"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.45.29279"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2011.01033.x"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20020508"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.164.1.2008022"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610770104"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2009.04.002"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2000.278.6.L1256"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2008-0053"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100109-104040"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa021660"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/5007"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1177\/44.7.8675988"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209144"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb05531.x"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004410100420"},{"key":"e_1_2_6_72_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.24.7432"},{"key":"e_1_2_6_73_1","doi-asserted-by":"publisher","DOI":"10.1159\/000253152"},{"key":"e_1_2_6_74_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008248"},{"key":"e_1_2_6_75_1","doi-asserted-by":"publisher","DOI":"10.2144\/00291bm09"},{"key":"e_1_2_6_76_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.034884"},{"key":"e_1_2_6_77_1","doi-asserted-by":"publisher","DOI":"10.3109\/01902141003690389"},{"key":"e_1_2_6_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00366-5"},{"key":"e_1_2_6_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.08.027"},{"key":"e_1_2_6_80_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7652557"},{"key":"e_1_2_6_81_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0540"},{"key":"e_1_2_6_82_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200507-1140OC"},{"key":"e_1_2_6_83_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00170.2003"},{"key":"e_1_2_6_84_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb05532.x"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.23773","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.23773","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T07:43:15Z","timestamp":1760341395000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.23773"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,23]]},"references-count":83,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1002\/dvdy.23773"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.23773","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,23]]}}}