{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:20:51Z","timestamp":1777666851251,"version":"3.51.4"},"reference-count":88,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,3,29]],"date-time":"2012-03-29T00:00:00Z","timestamp":1332979200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:styled-content>Background<\/jats:styled-content>: The basic helix\u2010loop\u2010helix (bHLH) transcription factor <jats:italic>Twist1<\/jats:italic> fulfills an essential function in neural crest cell formation, migration, and survival and is associated with the craniosynostic Saethre\u2010Chotzen syndrome in humans. However, its functions during mandibular development, when it may interact with other bHLH transcription factors like Hand2, are unknown because mice homozygous for the <jats:italic>Twist1<\/jats:italic> null mutation die in early embryogenesis. To determine the role of <jats:italic>Twist1<\/jats:italic> during mandibular development, we used the <jats:italic>Hand2\u2010Cre<\/jats:italic> transgene to conditionally inactivate the gene in the neural crest cells populating the mandibular pharyngeal arch. <jats:styled-content>Results<\/jats:styled-content>: The mutant mice exhibited a spectrum of craniofacial anomalies, including mandibular hypoplasia, altered middle ear development, and cleft palate. It appears that <jats:italic>Twist1<\/jats:italic> is essential for the survival of the neural crest cells involved in the development of the mandibular ramal elements. <jats:italic>Twist1<\/jats:italic> plays a role in molar development and cusp formation by participating in the reciprocal signaling needed for the formation of the enamel knot. This gene is also needed to control the ossification of the mandible, a redundant role shared with <jats:italic>Hand2<\/jats:italic>. <jats:styled-content>Conclusion<\/jats:styled-content>: Twist1, along with Hand2, is essential for the proximodistal patterning and development of the mandible and ossification. Developmental Dynamics, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.23776","type":"journal-article","created":{"date-parts":[[2012,3,12]],"date-time":"2012-03-12T14:17:12Z","timestamp":1331561832000},"page":"924-940","source":"Crossref","is-referenced-by-count":29,"title":["Specific inactivation of <i>Twist1<\/i> in the mandibular arch neural crest cells affects the development of the ramus and reveals interactions with hand2"],"prefix":"10.1002","volume":"241","author":[{"given":"Yanping","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evan L.","family":"Blackwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitchell T.","family":"McKnight","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory R.","family":"Knutsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy T.","family":"Vu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Bruno","family":"Ruest","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,3,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.062237ma"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.02.012"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22092"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.07.036"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.082747rb"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.056929"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.10156"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(04)00058-9"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.04.034"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.6.945"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.08.046"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20833"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.931701"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20332"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.6.686"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.05.499"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9527"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20717"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.03.037"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.18.4301"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2005.00485.x"},{"key":"e_1_2_6_23_1","first-page":"153","volume-title":"Topics in bone biology: bone and development","author":"D'Souza RN","year":"2010"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20796"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1075703"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2005.00487.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80257-3"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986708785908987"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M005888200"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1525"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702613200"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq890"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.029355"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/oso\/9780195118612.001.0001"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2008.04.003"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01705"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-6343.2009.01450.x"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0197-36"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/00125817-200211000-00011"},{"key":"e_1_2_6_40_1","first-page":"463","article-title":"Evidence for the role of the enamel knot as a control center in mammalian tooth cusp formation: non\u2010dividing cells express growth stimulating Fgf\u20104 gene","volume":"38","author":"Jernvall J","year":"1994","journal-title":"Int J Dev Biol"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.32458"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.07.011"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2005.01.015"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80258-5"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/6.7.1079"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1226(66)80044-9"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.08.045"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn920"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2005.02.001"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.12.041"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.04.024"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.10.055"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.09.061"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0730"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80259-7"},{"key":"e_1_2_6_56_1","first-page":"425","article-title":"The Pierre Robin syndrome: etiology and early treatment","author":"Poswillo D","year":"1967","journal-title":"Trans Int Conf Oral Surg"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.20.2523"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8556"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20501"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/tera.10022"},{"key":"e_1_2_6_61_1","doi-asserted-by":"crossref","first-page":"3005","DOI":"10.1242\/dev.121.9.3005","article-title":"Goosecoid is not an essential component of the mouse gastrula organizer but is required for craniofacial and rib development","volume":"121","author":"Rivera\u2010Perez JA","year":"1995","journal-title":"Development"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.33088"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.01.005"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110685200"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(03)00068-X"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01291"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051725"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.02.037"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2006.00525.x"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0699"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.06.004"},{"key":"e_1_2_6_72_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5267.1476"},{"key":"e_1_2_6_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.31360"},{"key":"e_1_2_6_74_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.2.217"},{"key":"e_1_2_6_75_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.23.3136"},{"key":"e_1_2_6_76_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5391.1136"},{"key":"e_1_2_6_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2010.07.009"},{"key":"e_1_2_6_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4773(95)00459-9"},{"key":"e_1_2_6_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.11.151"},{"key":"e_1_2_6_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.04.037"},{"key":"e_1_2_6_81_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.9.2917"},{"key":"e_1_2_6_82_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00337"},{"key":"e_1_2_6_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.06.006"},{"key":"e_1_2_6_84_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.24.4971"},{"key":"e_1_2_6_85_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21627"},{"key":"e_1_2_6_86_1","doi-asserted-by":"publisher","DOI":"10.3181\/0812-MR-365"},{"key":"e_1_2_6_87_1","doi-asserted-by":"crossref","first-page":"S35","DOI":"10.4103\/0970-0358.57185","article-title":"Cleft lip and palate genetics and application in early embryological development","volume":"42","author":"Yu W","year":"2009","journal-title":"Indian J Plast Surg"},{"key":"e_1_2_6_88_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.051789"},{"key":"e_1_2_6_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(02)00048-5"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.23776","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.23776","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T07:43:26Z","timestamp":1760341406000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.23776"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,29]]},"references-count":88,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1002\/dvdy.23776"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.23776","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,29]]}}}