{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T17:32:18Z","timestamp":1770571938808,"version":"3.49.0"},"reference-count":79,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,1,22]],"date-time":"2013-01-22T00:00:00Z","timestamp":1358812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:sec><jats:label\/><jats:p><jats:styled-content>Background:<\/jats:styled-content> Hearing restoration through hair cell regeneration will require revealing the dynamic interactions between proliferation and differentiation during development to avoid the limited viability of regenerated hair cells. <jats:styled-content style=\"fixed-case\"><jats:italic>Pax2\u2010Cre N\u2010Myc<\/jats:italic><\/jats:styled-content> conditional knockout (<jats:styled-content style=\"fixed-case\">CKO<\/jats:styled-content>) mice highlighted the need of <jats:styled-content style=\"fixed-case\"><jats:italic>N\u2010Myc<\/jats:italic><\/jats:styled-content> for proper neurosensory development and possible redundancy with <jats:styled-content style=\"fixed-case\"><jats:italic>L\u2010Myc<\/jats:italic><\/jats:styled-content>. The late\u2010onset hair cell death in the absence of early <jats:styled-content style=\"fixed-case\"><jats:italic>N\u2010Myc<\/jats:italic><\/jats:styled-content> expression could be due to mis\u2010regulation of genes necessary for neurosensory formation and maintenance, such as <jats:styled-content style=\"fixed-case\"><jats:italic>Neurod1, Atoh1, Pou4f3,<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>Barhl1<\/jats:italic><\/jats:styled-content>. <jats:styled-content>Results:<\/jats:styled-content> <jats:styled-content style=\"fixed-case\"><jats:italic>Pax2\u2010Cre N\u2010Myc L\u2010Myc<\/jats:italic><\/jats:styled-content> double <jats:styled-content style=\"fixed-case\">CKO<\/jats:styled-content> mice show that proliferation and differentiation are linked together through <jats:styled-content style=\"fixed-case\"><jats:italic>Myc<\/jats:italic><\/jats:styled-content> and in the absence of both <jats:styled-content style=\"fixed-case\"><jats:italic>Myc<\/jats:italic>s<\/jats:styled-content>, altered proliferation and differentiation result in morphologically abnormal ears. In particular, the organ of Corti apex is re\u2010patterned into a vestibular\u2010like organization and the base is truncated and fused with the saccule. <jats:styled-content>Conclusions:<\/jats:styled-content> These data indicate that therapeutic approaches to restore hair cells must take into account a dynamic interaction of proliferation and differentiation regulation of basic Helix\u2010Loop\u2010Helix transcription factors in attempts to stably replace lost cochlear hair cells. In addition, our data indicate that <jats:styled-content style=\"fixed-case\"><jats:italic>Myc<\/jats:italic><\/jats:styled-content> is an integral component of the evolutionary transformation process that resulted in the organ of Corti development. <jats:italic>Developmental Dynamics 242:132\u2013147, 2013<\/jats:italic>. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.23910","type":"journal-article","created":{"date-parts":[[2012,11,29]],"date-time":"2012-11-29T05:05:39Z","timestamp":1354165539000},"page":"132-147","source":"Crossref","is-referenced-by-count":34,"title":["Correct Timing of Proliferation and Differentiation is Necessary for Normal Inner Ear Development and Auditory Hair Cell Viability"],"prefix":"10.1002","volume":"242","author":[{"given":"Benjamin J.","family":"Kopecky","sequence":"first","affiliation":[{"name":"University of Iowa Department of Biology Iowa City Iowa"},{"name":"University of Iowa Carver College of Medicine Medical Scientist Training Program Iowa City Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Israt","family":"Jahan","sequence":"additional","affiliation":[{"name":"University of Iowa Department of Biology Iowa City Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"Fritzsch","sequence":"additional","affiliation":[{"name":"University of Iowa Department of Biology Iowa City Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.12.006"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.071670"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1121\/1.3212930"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5421.1837"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.08.055"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-213X-10-89"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.1993.tb00605.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1044\/1092-4388(2006\/095)"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01454-07"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.10.2495"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb976"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.09.074"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2011.01.005"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.10.4.14794"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808175105"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0785-11.2011"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-1704-0_11"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.103178jd"},{"key":"e_1_2_6_21_1","volume-title":"Hearing in vertebrates: a psychophysics databook","author":"Fay R","year":"1988"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/327153a0"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20502"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-010-0403-x"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2011.01.007"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.3766\/jaaa.21.7.3"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.078257"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10162-010-0211-x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2008.02.007"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02874"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011661"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030853"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3859-05.2006"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1995.0078"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9861(20010122)429:4<615::AID-CNE8>3.0.CO;2-F"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03487"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.07.004"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22620"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1037\/a0026896"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.23793"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2012.09.027"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2012.03673.x"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1273605"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4956-06.2007"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s101620010023"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0686-12.2012"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gep.2009.04.003"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01834"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2011.09.033"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.09.002"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20551"},{"key":"e_1_2_6_52_1","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1523\/JNEUROSCI.21-03-00974.2001","article-title":"Brief treatments with forskolin enhance s\u2010phase entry in balance epithelia from the ears of rats","volume":"21","author":"Montcouquiol M","year":"2001","journal-title":"J Neurosci"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-09-03327.1998"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2004.08.011"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-008-0709-2"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.4161\/cc.10.8.15301"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.20017"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2010.12.002"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030358"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10297"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20839"},{"key":"e_1_2_6_62_1","first-page":"41","article-title":"Stem cells and molecular strategies to restore hearing","volume":"50","author":"Pauley S","year":"2008","journal-title":"Panminerva Med"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.3233\/RNN-2010-0535"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-16-06125.2000"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2009.06.004"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.009118"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22381"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1303905"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5821-10.2011"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.moo.0000180919.68812.b9"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.5540790902"},{"key":"e_1_2_6_72_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510631103"},{"key":"e_1_2_6_73_1","doi-asserted-by":"publisher","DOI":"10.1159\/000057790"},{"key":"e_1_2_6_74_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708061105"},{"key":"e_1_2_6_75_1","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4612-2784-7","volume-title":"The evolutionary bilogy of hearing","author":"Webster D","year":"1992"},{"key":"e_1_2_6_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04849"},{"key":"e_1_2_6_77_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0953-816x.2001.01695.x"},{"key":"e_1_2_6_78_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2202-4-2"},{"key":"e_1_2_6_79_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msr132"},{"key":"e_1_2_6_80_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.17-01-00216.1997"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.23910","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.23910","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T18:28:15Z","timestamp":1760380095000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.23910"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,22]]},"references-count":79,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1002\/dvdy.23910"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.23910","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,22]]}}}