{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T22:35:17Z","timestamp":1792708517971,"version":"4.5.0"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,2,18]],"date-time":"2014-02-18T00:00:00Z","timestamp":1392681600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:p><jats:styled-content>Background:<\/jats:styled-content> Protein Phosphatase 2A (PP2A) function is controlled by regulatory subunits that modulate the activity of the catalytic subunit and direct the PP2A complex to specific intracellular locations. To study PP2A's role in signal transduction pathways that control growth and differentiation in vivo, a transgenic mouse lacking the B56\u03b3 regulatory subunit of PP2A was made. <jats:styled-content>Results:<\/jats:styled-content> Lack of PP2A activity specific to the PP2A\u2010B56\u03b3 holoenzyme, resulted in the formation of an incomplete ventricular septum and a decrease in the number of ventricular cardiomyocytes. During cardiac development, B56\u03b3 is expressed in the nucleus of \u03b1\u2010actinin\u2010positive cardiomyocytes that contain Z\u2010bands. The pattern of B56\u03b3 expression correlated with the cardiomyocyte apoptosis we observed in B56\u03b3\u2010deficient mice during mid to late gestation. In addition to the cardiac phenotypes, mice lacking B56\u03b3 have a decrease in locomotive coordination and gripping strength, indicating that B56\u03b3 has a role in controlling PP2A activity required for efficient neuromuscular function. <jats:styled-content>Conclusions:<\/jats:styled-content> PP2A\u2010B56\u03b3 activity is required for efficient cardiomyocyte maturation and survival. The PP2A B56\u03b3 regulatory subunit controls PP2A substrate specificity in vivo in a manner that cannot be fully compensated for by other B56 subunits. <jats:italic>Developmental Dynamics 243:778\u2013790, 2014<\/jats:italic>. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24111","type":"journal-article","created":{"date-parts":[[2014,1,15]],"date-time":"2014-01-15T01:42:00Z","timestamp":1389750120000},"page":"778-790","source":"Crossref","is-referenced-by-count":34,"title":["The protein phosphatase 2A B56\u03b3 regulatory subunit is required for heart development"],"prefix":"10.1002","volume":"243","author":[{"given":"Prajakta","family":"Varadkar","sequence":"first","affiliation":[{"name":"Division of Cellular and Gene Therapies Center for Biologics Evaluation and Research FDA Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daryl","family":"Despres","sequence":"additional","affiliation":[{"name":"Mouse Imaging Facility 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