{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T00:34:44Z","timestamp":1788568484677,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,3,19]],"date-time":"2014-03-19T00:00:00Z","timestamp":1395187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:p><jats:styled-content><jats:bold>Background:<\/jats:bold><\/jats:styled-content> Ectodysplasin (Eda) signaling is essential for the morphogenesis of several ectodermal appendages. <jats:styled-content><jats:bold>Results:<\/jats:bold><\/jats:styled-content> Here, we report a medaka mutant, <jats:italic>all\u2010fin less<\/jats:italic> (<jats:italic>afl<\/jats:italic>), which has a nonsense mutation in its <jats:italic>eda<\/jats:italic> gene. The adult <jats:italic>afl<\/jats:italic> fish displayed various abnormalities of its dermal skeleton, such as short and twisted fin rays, missing and abnormally shaped scales and teeth, and skull deformation. Focusing on the developing fin rays in the caudal region of <jats:italic>afl<\/jats:italic> larvae, we found that the fin rays did not elongate; although the initial formation of fin rays proceeded normally. Additionally, <jats:italic>eda<\/jats:italic> expression was lost, and the expression pattern of <jats:italic>edar<\/jats:italic>, the gene for the receptor of Eda, was different from wild\u2010type one. In vivo imaging of the double\u2010transgenic medaka expressing enhanced green fluorescent protein under control of the <jats:italic>edar<\/jats:italic> promoter and DsRed under control of the <jats:italic>osterix<\/jats:italic> promoter revealed that <jats:italic>edar<\/jats:italic> expression preceded that of <jats:italic>osterix<\/jats:italic> and that the <jats:italic>edar<\/jats:italic>\u2010expressing cells migrated in the direction of fin ray elongation, indicating that the Eda\/Edar signaling event precedes osteoblast differentiation. <jats:styled-content><jats:bold>Conclusions:<\/jats:bold><\/jats:styled-content> Our findings provide evidence that Eda signaling accompanied with the binding of Eda to Edar are essential for fin ray formation guided by cell migration. <jats:italic>Developmental Dynamics 243:765\u2013777, 2014<\/jats:italic>. \u00a9 2014 Wiley Periodicals, 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