{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:47:48Z","timestamp":1792788468155,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T00:00:00Z","timestamp":1400457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["UL1TR000427"],"award-info":[{"award-number":["UL1TR000427"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RR023916"],"award-info":[{"award-number":["RR023916"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001466","name":"American Urological Association Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001466","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:p><jats:styled-content>Background:<\/jats:styled-content> Homeostatic maintenance and repair of the bladder urothelium has been attributed to proliferation of keratin 5\u2010expressing basal cells (K5\u2010BC) with subsequent differentiation into superficial cells. Recent evidence, however, suggests that the intermediate cell layer harbors a population of progenitor cells. We use label\u2010retaining cell (LRC) methodology in conjunction with a clinically relevant model of uropathogenic <jats:italic>Escherichia coli<\/jats:italic> (UPEC)\u2010induced injury to characterize urothelial ontogeny during development and in response to diffuse urothelial injury. <jats:styled-content>Results:<\/jats:styled-content> In the developing urothelium, proliferating cells were dispersed throughout the K5\u2010BC and intermediate cells layers, becoming progressively concentrated in the K5\u2010BC layer with age. When 5\u2010bromo\u20102\u2010deoxyuridine (BrdU) was administered during urothelial development, LRCs in the adult were found within the K5\u2010BC, intermediate, and superficial cell layers, the location dependent upon time of labeling. UPEC inoculation resulted in loss of the superficial cell layer followed by robust proliferation of K5\u2010BCs and intermediate cells. LRCs within the K5\u2010BC and intermediate cell layers proliferated in response to injury. <jats:styled-content>Conclusions:<\/jats:styled-content> Urothelial development and regeneration following injury relies on proliferation of K5\u2010BC and intermediate cells. The existence and proliferation of LRCs within both the K5\u2010BC and intermediate cell layers suggests the presence of two populations of urothelial progenitor cells. <jats:italic>Developmental Dynamics 243::988\u2013998, 2014<\/jats:italic>. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24143","type":"journal-article","created":{"date-parts":[[2014,5,5]],"date-time":"2014-05-05T04:22:36Z","timestamp":1399263756000},"page":"988-998","source":"Crossref","is-referenced-by-count":60,"title":["A population of progenitor cells in the basal and intermediate layers of the murine bladder urothelium contributes to urothelial development and regeneration"],"prefix":"10.1002","volume":"243","author":[{"given":"Sara A.","family":"Colopy","sequence":"first","affiliation":[{"name":"Department of Surgical Sciences School of Veterinary Medicine, University of Wisconsin Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale E.","family":"Bjorling","sequence":"additional","affiliation":[{"name":"Department of Surgical Sciences School of Veterinary Medicine, University of Wisconsin Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William A.","family":"Mulligan","sequence":"additional","affiliation":[{"name":"Department of Urology School of Medicine, University of Wisconsin Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wade","family":"Bushman","sequence":"additional","affiliation":[{"name":"Department of Urology School of Medicine, University of Wisconsin Madison Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,5,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00341.2003"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1084550"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1998.4163"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2010.04.016"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urolonc.2009.04.019"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2005-0411"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2009.09.016"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.8.2035"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/s004180000240","article-title":"Postnatal restoration of the mouse urinary bladder urothelium","volume":"115","author":"Erman A","year":"2001","journal-title":"Histochem Cell Biol"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2006.07.001"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.07.017"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.26.1.25"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-185X.1975.tb01057.x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.6.2798-2802.1998"},{"key":"e_1_2_6_16_1","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1016\/S0022-5347(17)46083-6","article-title":"In vitro and in vivo adherence of uropathogenic Escherichia coli strains","volume":"135","author":"Hopkins WJ","year":"1986","journal-title":"J Urol"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00043.2002"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02899062"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(03)00005-0"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2490-12-17"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00327.2009"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.7A7269.2007"},{"key":"e_1_2_6_23_1","first-page":"1195","article-title":"Effects of a single dose of cyclophosphamide on various organs in the rat. 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