{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:11:40Z","timestamp":1790709100362,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,7,31]],"date-time":"2014-07-31T00:00:00Z","timestamp":1406764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01-AI081795"],"award-info":[{"award-number":["R01-AI081795"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Notre Dame Eck Insitute for Global Health","award":["Not applicable"],"award-info":[{"award-number":["Not applicable"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:sec><jats:label\/><jats:p><jats:bold><jats:styled-content>Background:<\/jats:styled-content><\/jats:bold> Despite the devastating impact of mosquito\u2010borne illnesses on human health, very little is known about mosquito developmental biology, including development of the mosquito visual system. Mosquitoes possess functional adult compound eyes as larvae, a trait that makes them an interesting model in which to study comparative developmental genetics. Here, we functionally characterize visual system development in the dengue and yellow fever vector mosquito <jats:italic>Aedes aegypti<\/jats:italic>, in which we use chitosan\/siRNA nanoparticles to target the axon guidance gene <jats:italic>semaphorin\u20101a<\/jats:italic> (<jats:italic>sema1a<\/jats:italic>). <jats:bold><jats:styled-content>Results:<\/jats:styled-content><\/jats:bold> Immunohistochemical analyses revealed the progression of visual sensory neuron targeting that results in generation of the retinotopic map in the mosquito optic lobe. Loss of <jats:italic>sema1a<\/jats:italic> function led to optic lobe phenotypes, including defective targeting of visual sensory neurons and failed formation of the retinotopic map. These <jats:italic>sema1a<\/jats:italic> knockdown phenotypes correlated with behavioral defects in larval photoavoidance. <jats:bold><jats:styled-content>Conclusions:<\/jats:styled-content><\/jats:bold> The results of this investigation indicate that Sema1a is required for optic lobe development in <jats:italic>A. aegypti<\/jats:italic> and highlight the behavioral importance of a functioning visual system in preadult mosquitoes. <jats:italic>Developmental Dynamics 243:1457\u20131469, 2014<\/jats:italic>. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.24168","type":"journal-article","created":{"date-parts":[[2014,7,17]],"date-time":"2014-07-17T05:03:15Z","timestamp":1405573395000},"page":"1457-1469","source":"Crossref","is-referenced-by-count":14,"title":["Role of <i>semaphorin\u20101a<\/i> in the developing visual system of the disease vector mosquito <i>Aedes aegypti<\/i>"],"prefix":"10.1002","volume":"243","author":[{"given":"Keshava","family":"Mysore","sequence":"first","affiliation":[{"name":"Department of Medical and Molecular Genetics Indiana University School of Medicine, Raclin\u2010Carmichael Hall South Bend Indiana"},{"name":"Eck Institute for Global Health, Brownson Hall University of Notre Dame Notre Dame Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen","family":"Flannery","sequence":"additional","affiliation":[{"name":"Eck Institute for Global Health, Brownson Hall University of Notre Dame Notre Dame Indiana"},{"name":"Department of Biological Sciences, Galvin Life Sciences University of Notre Dame Notre Dame Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew T.","family":"Leming","sequence":"additional","affiliation":[{"name":"Eck Institute for Global Health, Brownson Hall University of Notre Dame Notre Dame Indiana"},{"name":"Department of Biological Sciences, Galvin Life Sciences University of Notre Dame Notre Dame Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Tomchaney","sequence":"additional","affiliation":[{"name":"Eck Institute for Global Health, Brownson Hall University of Notre Dame Notre Dame Indiana"},{"name":"Department of Biological Sciences, Galvin Life Sciences University of Notre Dame Notre Dame Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Shi","sequence":"additional","affiliation":[{"name":"Eck Institute for Global Health, Brownson Hall University of Notre Dame Notre Dame Indiana"},{"name":"Department of Biological Sciences, Galvin Life Sciences University of Notre Dame Notre Dame Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longhua","family":"Sun","sequence":"additional","affiliation":[{"name":"Eck Institute for Global Health, Brownson Hall University of Notre Dame Notre Dame Indiana"},{"name":"Department of Biological Sciences, Galvin Life Sciences University of Notre Dame Notre Dame Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph E.","family":"O'Tousa","sequence":"additional","affiliation":[{"name":"Eck Institute for Global Health, Brownson Hall University of Notre Dame Notre Dame Indiana"},{"name":"Department of Biological Sciences, Galvin Life Sciences University of Notre Dame Notre Dame Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Severson","sequence":"additional","affiliation":[{"name":"Department of Medical and Molecular Genetics Indiana University School of Medicine, Raclin\u2010Carmichael Hall South Bend Indiana"},{"name":"Eck Institute for Global Health, Brownson Hall University of Notre Dame Notre Dame Indiana"},{"name":"Department of Biological Sciences, Galvin Life Sciences University of Notre Dame Notre Dame Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly","family":"Duman\u2010Scheel","sequence":"additional","affiliation":[{"name":"Department of Medical and Molecular Genetics Indiana University School of Medicine, Raclin\u2010Carmichael Hall South Bend Indiana"},{"name":"Eck Institute for Global Health, Brownson Hall University of Notre Dame Notre Dame Indiana"},{"name":"Department of Biological Sciences, Galvin Life Sciences University of Notre Dame Notre Dame 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