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Furthermore, we identified a temporal expression pattern of early UB genes during UB epithelium development in which gene expression was detected at early developmental stages and became undetectable by embryonic day 14.5. In contrast, expression of early UB genes persisted at later stages in the <jats:italic>Dicer<\/jats:italic> mutant UB epithelium and increased at early stages. Our bioinformatic analysis of the abnormally persistently expressed early genes in the <jats:italic>Dicer<\/jats:italic> mutant UB epithelium showed significant enrichment of the <jats:italic>let\u20107<\/jats:italic> family miRNA targets. We further identified a temporal expression pattern of <jats:italic>let\u20107<\/jats:italic> miRNAs in the UB epithelium that is anti\u2010parallel to that of some early UB genes during kidney development. <jats:bold><jats:styled-content>Conclusions:<\/jats:styled-content><\/jats:bold> We propose a model in which the <jats:italic>let\u20107<\/jats:italic> family miRNAs silence the expression of a subset of early genes in the UB epithelium at later developmental stages to promote collecting duct differentiation. <jats:italic>Developmental Dynamics 244:444\u2013456, 2015<\/jats:italic>. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24221","type":"journal-article","created":{"date-parts":[[2014,11,5]],"date-time":"2014-11-05T02:11:50Z","timestamp":1415153510000},"page":"444-456","source":"Crossref","is-referenced-by-count":9,"title":["microRNA\u2010dependent temporal gene expression in the ureteric bud epithelium during mammalian kidney development"],"prefix":"10.1002","volume":"244","author":[{"given":"Vidya K.","family":"Nagalakshmi","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Child Health Research Center University of Virginia School of Medicine Charlottesville Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Volkhard","family":"Lindner","sequence":"additional","affiliation":[{"name":"Center for Molecular Medicine, Maine Medical Center Research Institute Scarborough Maine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy","family":"Wessels","sequence":"additional","affiliation":[{"name":"Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston South Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Child Health Research Center University of Virginia School of Medicine Charlottesville Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/1073858413497265"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2011.04.003"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.280.3.F437"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms141123086"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-013-1000-x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI20665"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1677\/ERC-09-0184"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2008.07.001"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20143"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI73351"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2010.106"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00802-13"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20452"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2013.98"},{"issue":"2011","key":"e_1_2_6_16_1","doi-asserted-by":"crossref","first-page":"pdb:prot5613","DOI":"10.1101\/pdb.prot5613","article-title":"Dissection of embryonic mouse kidney, culture in vitro, and imaging of the developing organ","author":"Costantini F","year":"2011","journal-title":"Cold Spring Harb Protoc"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvn151"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2014.00005"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2621"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.05.029"},{"key":"e_1_2_6_21_1","unstructured":"GuoQ WangY TripathiP MandaKR MukherjeeM ChakladerM AustinPF SurendranK ChenF.2014. 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