{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T22:41:39Z","timestamp":1762641699263,"version":"build-2065373602"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,12,15]],"date-time":"2014-12-15T00:00:00Z","timestamp":1418601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:p><jats:styled-content>Background:<\/jats:styled-content> The rodent salivary gland is not fully developed at birth and the cellular definitive differentiation takes place postnatally. However, little is known about its molecular mechanism. <jats:bold><jats:styled-content>Results:<\/jats:styled-content><\/jats:bold> Here we provide the loss\u2010of\u2010function genetic evidence that Runx signaling affects postnatal development of the submandibular gland (SMG). Core binding factor \u03b2 (Cbfb) is a cotranscription factor which forms a heterodimer with Runx proteins. <jats:italic>Cbfb<\/jats:italic> was specifically expressed in the duct epithelium, specifically in the SMG. Epithelial Cbfb deficiency resulted in decrease in the size of the SMG and in the saliva secretion on postnatal day 35. The <jats:italic>Cbfb<\/jats:italic> mutant SMG specifically exhibited involution of the granular convoluted tubules (GCT), with a down\u2010regulated expression of its marker genes, such as <jats:italic>Klk1<\/jats:italic>, <jats:italic>Ngf<\/jats:italic>, and <jats:italic>Egf<\/jats:italic>. The induction of GCT is under the control of androgens, and the <jats:italic>Cbfb<\/jats:italic> mutant SMG demonstrated down\u2010regulated expression of <jats:italic>Crisp3<\/jats:italic>, an androgen\u2010dependent transcript. Because the circulating testosterone or tissue dihydrotestosterone levels were not affected in the <jats:italic>Cbfb<\/jats:italic> mutants, it appears that Runx\/Cbfb signaling regulate androgen receptor pathway, but does not affect the circulating testosterone levels or the enzymatic conversion to DHT. <jats:bold><jats:styled-content>Conclusions:<\/jats:styled-content><\/jats:bold> Runx signaling is important in the postnatal development of androgen\u2010dependent GCT in the SMG. <jats:italic>Developmental Dynamics 244:488\u2013496, 2015<\/jats:italic>. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24231","type":"journal-article","created":{"date-parts":[[2014,11,20]],"date-time":"2014-11-20T02:08:35Z","timestamp":1416449315000},"page":"488-496","source":"Crossref","is-referenced-by-count":10,"title":["Runx\/Cbfb signaling regulates postnatal development of granular convoluted tubule in the mouse submandibular gland"],"prefix":"10.1002","volume":"244","author":[{"given":"Md. Nurul","family":"Islam","sequence":"first","affiliation":[{"name":"Department of Orthodontics Science of Functional Recovery and Reconstruction Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinsuke","family":"Itoh","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Science of Functional Recovery and Reconstruction Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama Japan"},{"name":"Department of Orthodontics and Dentofacial Orthopedics Osaka University Graduate School of Dentistry Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Yanagita","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Okayama University Hospital Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumi","family":"Sumiyoshi","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Okayama University Hospital Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoru","family":"Hayano","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Science of Functional Recovery and Reconstruction Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koh\u2010Ichi","family":"Kuremoto","sequence":"additional","affiliation":[{"name":"Department of Advanced Prosthodontics Hiroshima University Graduate School of Biomedical &amp; Health Sciences Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Kurosaka","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Science of Functional Recovery and Reconstruction Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama Japan"},{"name":"Department of Orthodontics and Dentofacial Orthopedics Osaka University Graduate School of Dentistry Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadashi","family":"Honjo","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Science of Functional Recovery and Reconstruction Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriaki","family":"Kawanabe","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Science of Functional Recovery and Reconstruction Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Kamioka","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Science of Functional Recovery and Reconstruction Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayoshi","family":"Sakai","sequence":"additional","affiliation":[{"name":"Department of Oral\u2010facial Disorders Osaka University Graduate School of Dentistry Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naozumi","family":"Ishimaru","sequence":"additional","affiliation":[{"name":"Department of Oral Molecular Pathology Institute of Health Biosciences, The University of Tokushima Graduate School Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichiro","family":"Taniuchi","sequence":"additional","affiliation":[{"name":"Laboratory for Transcriptional Regulation RIKEN Research Center for Allergy and Immunology Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Yamashiro","sequence":"additional","affiliation":[{"name":"Department of Orthodontics Science of Functional Recovery and Reconstruction Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama Japan"},{"name":"Department of Orthodontics and Dentofacial Orthopedics Osaka University Graduate School of Dentistry Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,12,15]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(66)80004-7"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00218202"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)60101-1"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.22.4775"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80257-3"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1070270102"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-08-3280"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.133.1.192"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.17.4694"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn925"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(07)99002-8"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80258-5"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2010.01669.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.722"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf370"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(01)00537-8"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00690-6"},{"key":"e_1_2_5_19_1","first-page":"205","article-title":"Differential actions of testosterone and its metabolites on mice submandibular gland","volume":"13","author":"Minetti CA","year":"1985","journal-title":"J Biol Buccale"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(92)90207-M"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345750540012601"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20062456"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.012799"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.23804"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1159\/000097197"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.22.12359"},{"issue":"3","key":"e_1_2_5_27_1","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1042\/bj3090831","article-title":"Isolation and characterization of the androgen\u2010dependent mouse cysteine\u2010rich secretory protein\u20103 (CRISP\u20103) gene","volume":"309","author":"Schwidetzky U","year":"1995","journal-title":"Biochem J"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0420.1996.tb00594.x"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2007.01.006"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.13-11791"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81389-6"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(03)00101-1"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.24231","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.24231","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T10:53:32Z","timestamp":1760352812000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.24231"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,15]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["10.1002\/dvdy.24231"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.24231","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"type":"print","value":"1058-8388"},{"type":"electronic","value":"1097-0177"}],"subject":[],"published":{"date-parts":[[2014,12,15]]}}}