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However, this is often a time\u2010consuming process where the quantification of cardiac defects can be difficult due to problems with accurate section registration. Here we demonstrate the advantages of using optical coherence tomography, a comparatively new and rising technology, to phenotype avian embryo hearts in a model of fetal alcohol syndrome where a binge\u2010like quantity of alcohol\/ethanol was introduced at gastrulation. <jats:bold>Results:<\/jats:bold> The rapid, consistent imaging protocols allowed for the immediate identification of cardiac anomalies, including ventricular septal defects and misaligned\/missing vessels. Interventricular septum thicknesses and vessel diameters for three of the five outflow arteries were also significantly reduced. Outflow and atrioventricular valves were segmented using image processing software and had significantly reduced volumes compared to controls. This is the first study to our knowledge that has 3D reconstructed the late\u2010stage cardiac valves in precise detail to examine their morphology and dimensions. <jats:bold>Conclusions:<\/jats:bold> We believe, therefore, that optical coherence tomography, with its ability to rapidly image and quantify tiny embryonic structures in high resolution, will serve as an excellent and cost\u2010effective preliminary screening tool for developmental biologists working with a variety of experimental\/disease models. <jats:italic>Developmental Dynamics 244:607\u2013618, 2015<\/jats:italic>. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24246","type":"journal-article","created":{"date-parts":[[2014,12,27]],"date-time":"2014-12-27T00:43:22Z","timestamp":1419641002000},"page":"607-618","source":"Crossref","is-referenced-by-count":26,"title":["Using optical coherence tomography to rapidly phenotype and quantify congenital heart defects associated with prenatal alcohol 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