{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:00:35Z","timestamp":1791327635412,"version":"4.3.1"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,4,8]],"date-time":"2015-04-08T00:00:00Z","timestamp":1428451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:p>\n                    <jats:styled-content>Background:<\/jats:styled-content>\n                    GLI2, a zinc finger transcription factor, mediates Sonic hedgehog signaling, a critical pathway in vertebrate embryogenesis. GLI2 has been implicated in diverse set of embryonic developmental processes, including patterning of central nervous system and limbs. In humans, mutations in\n                    <jats:italic>GLI2<\/jats:italic>\n                    are associated with several developmental defects, including holoprosencephaly and polydactyly.\n                    <jats:styled-content>Results:<\/jats:styled-content>\n                    Here, we demonstrate in transient transgenic zebrafish assays, the potential of a subset of tetrapod\u2010teleost conserved non\u2010coding elements (CNEs) residing within human\n                    <jats:italic>GLI2<\/jats:italic>\n                    intronic intervals to induce reporter gene expression at known regions of endogenous GLI2 transcription. The regulatory activities of these elements are observed in several embryonic domains, including neural tube and pectoral fin. Moreover, our data reveal an overlapping expression profile of duplicated copies of an enhancer during zebrafish evolution.\n                    <jats:styled-content>Conclusions:<\/jats:styled-content>\n                    Our data suggest that during vertebrate history\n                    <jats:italic>GLI2<\/jats:italic>\n                    acquired a high level of complexity in the genetic mechanisms regulating its expression during spatiotemporal patterning of the central nervous system (CNS) and limbs.\n                    <jats:italic>Developmental Dynamics 244:681\u2013692, 2015<\/jats:italic>\n                    . \u00a9 2015 Wiley Periodicals, Inc.\n                  <\/jats:p>","DOI":"10.1002\/dvdy.24266","type":"journal-article","created":{"date-parts":[[2015,2,25]],"date-time":"2015-02-25T17:36:16Z","timestamp":1424885776000},"page":"681-692","source":"Crossref","is-referenced-by-count":9,"title":["<i>Cis<\/i>\n                    \u2010regulatory control of human GLI2 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