{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T23:38:11Z","timestamp":1790552291703,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,6,18]],"date-time":"2015-06-18T00:00:00Z","timestamp":1434585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1 DK064640"],"award-info":[{"award-number":["RO1 DK064640"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:p><jats:bold><jats:styled-content>Background:<\/jats:styled-content><\/jats:bold> Specification of the metanephric mesenchyme is a central step of kidney development as this mesenchyme promotes nephric duct induction to form a ureteric bud near its caudal end. Before ureteric bud formation, the caudal nephric duct swells to form a pseudostratified epithelial domain that later emerges as the tip of the bud. However, the signals that promote the formation of the transient epithelial domain remain unclear. Here, we investigated the early roles of the mesenchymal factor Six family and its cofactor Eya on the initial induction of nephric duct development.<\/jats:p><jats:p><jats:bold><jats:styled-content>Results:<\/jats:styled-content><\/jats:bold> The nephrogenic progenitor population is initially present but significantly reduced in mice lacking both <jats:italic>Six1<\/jats:italic> and <jats:italic>Six4<\/jats:italic> and undertakes an abnormal cell death pathway to be completely eliminated by \u223cE10.5\u2013E11.0, similar to that observed in <jats:italic>Eya1<jats:sup>\u2010\/\u2010<\/jats:sup><\/jats:italic> embryos. Consequently, the nephric duct fails to be induced to undergo normal proliferation to pseudostratify and form the ureteric bud in <jats:italic>Six1<jats:sup>\u2010\/\u2010<\/jats:sup>;Six4<jats:sup>\u2010\/\u2010<\/jats:sup><\/jats:italic> or <jats:italic>Eya1<jats:sup>\u2010\/\u2010<\/jats:sup><\/jats:italic> embryos.<\/jats:p><jats:p><jats:bold><jats:styled-content>Conclusions:<\/jats:styled-content><\/jats:bold> Our data support a model where Eya\u2010Six may form a complex to regulate nephron progenitor cell development before metanephric specification and are critical mesenchymal factors for inducing nephric duct development. <jats:italic>Developmental Dynamics 244:866\u2013873, 2015<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24282","type":"journal-article","created":{"date-parts":[[2015,4,22]],"date-time":"2015-04-22T04:39:29Z","timestamp":1429677569000},"page":"866-873","source":"Crossref","is-referenced-by-count":24,"title":["Eya\u2010six are necessary for survival of nephrogenic cord progenitors and inducing nephric duct development before ureteric bud formation"],"prefix":"10.1002","volume":"244","author":[{"given":"Jinshu","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Genetics and Genomic Sciences Icahn School of Medicine at Mount Sinai New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pin\u2010Xian","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genomic Sciences Icahn School of Medicine at Mount Sinai New York New York"},{"name":"Department of Developmental and Regenerative Biology Icahn School of Medicine at Mount Sinai New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,6,18]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2011.12.006"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.071670"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.24.2775"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.07.013"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.2006.00106.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.4161\/org.6.4.12680"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20357"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000049905"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.22.010305.104340"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.034876"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01773"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.14.6253-6267.2004"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.02442"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2007.01.002"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.022830"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.09.010"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2011.01.027"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.10.3343-3350.2001"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.05.029"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1987.tb00176.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00849241"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg842"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.09.024"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.103283"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2014.10.015"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/12722"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00536"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00628"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2005.12.015"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01437"}],"container-title":["Developmental Dynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvdy.24282","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvdy.24282","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T17:00:59Z","timestamp":1760374859000},"score":1,"resource":{"primary":{"URL":"https:\/\/anatomypubs.onlinelibrary.wiley.com\/doi\/10.1002\/dvdy.24282"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,18]]},"references-count":30,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["10.1002\/dvdy.24282"],"URL":"https:\/\/doi.org\/10.1002\/dvdy.24282","archive":["Portico"],"relation":{},"ISSN":["1058-8388","1097-0177"],"issn-type":[{"value":"1058-8388","type":"print"},{"value":"1097-0177","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,18]]}}}