{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T20:15:32Z","timestamp":1789935332868,"version":"4.0.1"},"reference-count":72,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,7,29]],"date-time":"2015-07-29T00:00:00Z","timestamp":1438128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R01DK076647"],"award-info":[{"award-number":["R01DK076647"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["F30DK091992"],"award-info":[{"award-number":["F30DK091992"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p><jats:styled-content>Background:<\/jats:styled-content> As the pituitary gland develops, signals from the hypothalamus are necessary for pituitary induction and expansion. Little is known about the control of cues that regulate early signaling between the two structures. Ligands and receptors of the Notch signaling pathway are found in both the hypothalamus and Rathke's pouch. The downstream Notch effector gene <jats:italic>Hes1<\/jats:italic> is required for proper pituitary formation; however, these effects could be due to the action of <jats:italic>Hes1<\/jats:italic> in the hypothalamus, Rathke's pouch, or both. To determine the contribution of hypothalamic Notch signaling to pituitary organogenesis, we used mice with loss and gain of Notch function within the developing hypothalamus. <jats:styled-content>Results:<\/jats:styled-content> We demonstrate that loss of Notch signaling by conditional deletion of <jats:italic>Rbpj<\/jats:italic> in the hypothalamus does not affect expression of <jats:italic>Hes1<\/jats:italic> within the posterior hypothalamus or expression of <jats:italic>Hes5<\/jats:italic>. In contrast, expression of activated Notch within the hypothalamus results in ectopic <jats:italic>Hes5<\/jats:italic> expression and increased <jats:italic>Hes1<\/jats:italic> expression, which is sufficient to disrupt pituitary development and postnatal expansion. <jats:styled-content>Conclusions:<\/jats:styled-content> Taken together, our results indicate that <jats:italic>Rbpj<\/jats:italic>\u2010dependent Notch signaling within the developing hypothalamus is not necessary for pituitary development, but persistent Notch signaling and ectopic <jats:italic>Hes5<\/jats:italic> expression in hypothalamic progenitors affects pituitary induction and expansion. <jats:italic>Developmental Dynamics 244:921\u2013934, 2015<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24283","type":"journal-article","created":{"date-parts":[[2015,4,22]],"date-time":"2015-04-22T04:39:13Z","timestamp":1429677553000},"page":"921-934","source":"Crossref","is-referenced-by-count":16,"title":["Persistent expression of activated notch in the developing hypothalamus affects survival of pituitary progenitors and alters pituitary structure"],"prefix":"10.1002","volume":"244","author":[{"given":"Paven K.","family":"Aujla","sequence":"first","affiliation":[{"name":"Neuroscience Program University of Illinois at Urbana\u2010Champaign Urbana Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vedran","family":"Bogdanovic","sequence":"additional","affiliation":[{"name":"Department of Molecular and Integrative Physiology University of Illinois at Urbana\u2010Champaign Urbana Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George 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