{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T04:47:44Z","timestamp":1790484464689,"version":"4.1.0"},"reference-count":73,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,7,2]],"date-time":"2015-07-02T00:00:00Z","timestamp":1435795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002946","name":"Deutsches Zentrum f\u00fcr Luft- und Raumfahrt","doi-asserted-by":"publisher","award":["FKZ 50WBO257"],"award-info":[{"award-number":["FKZ 50WBO257"]}],"id":[{"id":"10.13039\/501100002946","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002790","name":"Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["DG 328376"],"award-info":[{"award-number":["DG 328376"]}],"id":[{"id":"10.13039\/501100002790","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p><jats:bold><jats:styled-content>Background:<\/jats:styled-content><\/jats:bold> Bones are mainly composed of calcium hydroxyapatite and a proteinous matrix. In this study, we focus on the bone matrix proteins, the fish osteopontin orthologous protein (osteopontin\u2010like protein; OP\u2010L) and SPARC, because the current knowledge regarding their expression is fragmentary or contradictory. <jats:bold><jats:styled-content>Results:<\/jats:styled-content><\/jats:bold> We first provide a comprehensive and detailed description of skeletal development in the cichlid fish <jats:italic>Oreochromis mossambicus<\/jats:italic>. Following this, we analyzed the expression pattern of OP\u2010L and SPARC in detail during development. OP\u2010L expression was only found in tissues that undergo ossification (i.e., developing bones and teeth). Furthermore, we show that there is a fundamental difference in cartilage formation of the splanchnocranium and all other cartilages, concerning SPARC expression. Significantly, we show that the initial calcification of cranial bones occurs simultaneously with the expression of OP\u2010L and SPARC in the osteoblast\u2010like cells, which appear early in development. <jats:bold><jats:styled-content>Conclusions:<\/jats:styled-content><\/jats:bold> The difference in SPARC expression during chondrogenesis of the splanchnocranium is likely based on its different evolutionary history compared with the dermatocranium and chondrocranium. Moreover, our results suggest a co\u2010occurrence of the initial calcium deposition and bone matrix protein expression during osteogenesis. Overall, this study enhances our understanding of fish skeletal development and evolution. <jats:italic>Developmental Dynamics 244:955\u2013972, 2015<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24293","type":"journal-article","created":{"date-parts":[[2015,5,22]],"date-time":"2015-05-22T05:41:04Z","timestamp":1432273264000},"page":"955-972","source":"Crossref","is-referenced-by-count":15,"title":["Expression of SPARC and the osteopontin\u2010like protein during skeletal development in the cichlid fish <i>Oreochromis mossambicus<\/i>"],"prefix":"10.1002","volume":"244","author":[{"given":"Jochen","family":"Weigele","sequence":"first","affiliation":[{"name":"Zoological Institute University of Stuttgart\u2010Hohenheim Stuttgart Germany"},{"name":"Department of Biology Mount Saint Vincent University Halifax Nova Scotia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara A.","family":"Franz\u2010Odendaal","sequence":"additional","affiliation":[{"name":"Department of Biology Mount Saint Vincent University Halifax Nova Scotia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Hilbig","sequence":"additional","affiliation":[{"name":"Zoological Institute University of Stuttgart\u2010Hohenheim Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,7,2]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"1","article-title":"The early morphogenetic development of the chichlid fish, Oreochromis mossambicus (Perciformes, Teleostei)","volume":"231","author":"Anken RA","year":"1993","journal-title":"Zool Anz"},{"key":"e_1_2_5_3_1","first-page":"1","article-title":"Development of the dermal bones in the skull of lepidosteus osseus","volume":"83","author":"Aumonier FJ","year":"1941","journal-title":"J Micro Sci"},{"key":"e_1_2_5_4_1","first-page":"166","article-title":"Early development of the cephalic skeleton in the turbot","volume":"52","author":"Aumonier FJ","year":"1941","journal-title":"J Fish Biol"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20619"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2978"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.140021997"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.asr.2003.09.044"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(97)00125-1"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02090-7"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1030790100"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540205000101"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI16804"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(00)00133-5"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650090609"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008208909002412"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2002-221044"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.7.15.8262332"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0030254"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2004.11.018"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2007.05972.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2320715"},{"key":"e_1_2_5_23_1","first-page":"641","volume-title":"The ciclidfishes of the great lakes of Africa. 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