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Molecular mechanisms controlling SHF\/DMP development include the Bone Morphogenetic Protein and Wnt\/\u03b2\u2010catenin signaling pathways. Reduced expression of components in these pathways leads to inhibition of proliferation of the SHF\/DMP precursor population and failure of the DMP to develop. While the Sonic Hedgehog (Shh) pathway has also been demonstrated to be critically important for SHF\/DMP development and atrioventricular septation, its role in the regulation of SHF proliferation is contentious. <jats:bold><jats:styled-content>Results:<\/jats:styled-content><\/jats:bold> Tissue\u2010specific deletion of the Shh receptor Smoothened from the SHF resulted in compromised DMP formation and atrioventricular septal defects (AVSDs). Immunohistochemical analysis at critical stages of DMP development showed significant proliferation defect as well as reduction in levels of the Wnt\/\u03b2\u2010catenin pathway\u2010intermediates \u03b2\u2010catenin, Lef1, and Axin2. To determine whether the defects seen in the conditional Smoothened knock\u2010out mouse could be attributed to reduced Wnt\/\u03b2\u2010catenin signaling, LiCl, a pharmacological activator of this Wnt\/\u03b2\u2010catenin pathway, was administered. This resulted in restoration of proliferation and partial rescue of the AVSD phenotype. <jats:bold><jats:styled-content>Conclusions:<\/jats:styled-content><\/jats:bold> The data presented suggest that the Wnt\/\u03b2\u2010catenin pathway interact with the Shh pathway in the regulation of SHF\/DMP\u2010precursor proliferation and, hence, the development of the DMP. <jats:italic>Developmental Dynamics 245:103\u2013113, 2016<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24339","type":"journal-article","created":{"date-parts":[[2015,8,22]],"date-time":"2015-08-22T09:41:26Z","timestamp":1440236486000},"page":"103-113","source":"Crossref","is-referenced-by-count":49,"title":["Wnt\/\u03b2\u2010catenin and sonic hedgehog pathways interact in the regulation of the development of the dorsal mesenchymal protrusion"],"prefix":"10.1002","volume":"245","author":[{"given":"Laura E.","family":"Briggs","sequence":"first","affiliation":[{"name":"Department of 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