{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T10:59:25Z","timestamp":1774263565609,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,1,8]],"date-time":"2016-01-08T00:00:00Z","timestamp":1452211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:p><jats:styled-content><jats:bold>Background:<\/jats:bold><\/jats:styled-content> It is widely accepted that, during the development of testes in the mammalian embryo, male germ cells are influenced by signals from the surrounding somatic cells, but not vice versa, so that germ cells are dispensable for the formation of testes. <jats:styled-content><jats:bold>Results:<\/jats:bold><\/jats:styled-content> We now demonstrate that development of the mouse fetal testis is compromised in the absence of germ cells. Using two\u2010 and three\u2010dimensional imaging techniques, we reveal that W<jats:sup>e<\/jats:sup>\/W<jats:sup>e<\/jats:sup> mutant testes devoid of germ cells have misshapen and poorly organized cords. We also found that mutant gonads have fewer Sertoli cells than normal and that the Leydig cells express key markers at higher than normal levels. <jats:styled-content><jats:bold>Conclusions:<\/jats:bold><\/jats:styled-content> These observations point to the existence of germ cell\u2010derived signals that directly or indirectly affect the Sertoli and Leydig cell populations, and provide a new paradigm for the organogenesis of the mammalian testes. <jats:italic>Developmental Dynamics 245:433\u2013444, 2016<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24371","type":"journal-article","created":{"date-parts":[[2015,12,6]],"date-time":"2015-12-06T02:47:16Z","timestamp":1449370036000},"page":"433-444","source":"Crossref","is-referenced-by-count":16,"title":["Germ cells influence cord formation and leydig cell gene expression during mouse testis 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