{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T11:10:44Z","timestamp":1772190644307,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,2,5]],"date-time":"2016-02-05T00:00:00Z","timestamp":1454630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"PAPIIT-UNAM","award":["IN208512"],"award-info":[{"award-number":["IN208512"]}]},{"name":"CONACyT","award":["166046"],"award-info":[{"award-number":["166046"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:p><jats:styled-content>Background:<\/jats:styled-content> Scarb2 or Limp2 belong to a subfamily of Scavenger receptors described as lysosomal transmembrane glycosylated receptors, that are mutated in the human syndrome AMRF (action myoclonus\u2010renal failure). The zebrafish insertional mutant <jats:italic>scarb2a<\/jats:italic><jats:sup><jats:italic>hi1463Tg<\/jats:italic><\/jats:sup> has notochord defects, the notochord is a defining feature of chordates running along the center of the longitudinal axis and it is essential for forming the spinal column in all vertebrates. <jats:styled-content>Results:<\/jats:styled-content> There are three paralogous <jats:italic>scarb2<\/jats:italic> genes in zebrafish; <jats:italic>scarb2a, scarb2b<\/jats:italic>, and <jats:italic>scarb2c<\/jats:italic>. Both Scarb2a and Scarb2b proteins lack the classical di\u2010leucine motif. We found that <jats:italic>scarb2a<\/jats:italic><jats:sup><jats:italic>hi1463Tg<\/jats:italic><\/jats:sup> homozygous zebrafish embryos have a null mutation impairing vacuole formation in the notochord and simultaneously disrupting proper formation of the basement membrane resulting in its thickening at the ventral side of the notochord, which may be the cause for the anomalous upward bending observed in the trunk. Through whole\u2010mount in situ hybridization, we detected <jats:italic>scarb2a<\/jats:italic> mRNA expression in the notochord and in the brain early in development. However, it is puzzling that <jats:italic>scarb2a<\/jats:italic> notochord mRNA expression is short\u2010lived in the presumptive notochord and precedes the complete differentiation of the notochord. <jats:styled-content>Conclusions:<\/jats:styled-content> This work describes a novel function for the Scarb2 receptor as an essential glycoprotein for notochord development. <jats:italic>Developmental Dynamics 245:508\u2013519, 2016<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24383","type":"journal-article","created":{"date-parts":[[2016,1,8]],"date-time":"2016-01-08T00:29:15Z","timestamp":1452212955000},"page":"508-519","source":"Crossref","is-referenced-by-count":13,"title":["Zebrafish <i>scarb2a<\/i> insertional mutant reveals a novel function for the Scarb2\/Limp2 receptor in notochord development"],"prefix":"10.1002","volume":"245","author":[{"given":"Abigail","family":"Diaz\u2010Tellez","sequence":"first","affiliation":[{"name":"EvoDevo Lab, Unidad de Sistemas Arrecifales, Instituto de Ciencias del Mar y Limnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico Puerto Morelos Quintana Roo M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia","family":"Zampedri","sequence":"additional","affiliation":[{"name":"EvoDevo Lab, Unidad de Sistemas Arrecifales, Instituto de Ciencias del Mar y Limnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico Puerto Morelos Quintana Roo M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose L.","family":"Ramos\u2010Balderas","sequence":"additional","affiliation":[{"name":"Instituto de Fisiolog\u00eda Celular Universidad Nacional Aut\u00f3noma de M\u00e9xico  D.F. M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Garc\u00eda\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto de Fisiolog\u00eda Celular Universidad Nacional Aut\u00f3noma de M\u00e9xico  D.F. M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ernesto","family":"Maldonado","sequence":"additional","affiliation":[{"name":"EvoDevo Lab, Unidad de Sistemas Arrecifales, Instituto de Ciencias del Mar y Limnolog\u00eda Universidad Nacional Aut\u00f3noma de M\u00e9xico Puerto Morelos Quintana Roo 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