{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T16:11:33Z","timestamp":1787674293627,"version":"build-2784847793"},"reference-count":44,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,8,25]],"date-time":"2016-08-25T00:00:00Z","timestamp":1472083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["RO1 HL121382 (CBK)"],"award-info":[{"award-number":["RO1 HL121382 (CBK)"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Institutes of Health-National Institute of General Medical Sciences","award":["P30 GM103342"],"award-info":[{"award-number":["P30 GM103342"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:p><jats:styled-content><jats:bold>Background<\/jats:bold>:<\/jats:styled-content> There are many patients that exhibit connective tissue related cardiac malformations but do not have mutations in collagen genes. The Small Leucine Rich Proteoglycans (SLRP) fibromodulin (FMOD) and lumican (LUM) bind collagen and regulate fibril assembly in other biological contexts. <jats:styled-content><jats:bold>Results<\/jats:bold>:<\/jats:styled-content> FMOD deficient mice and double deficient FMOD; LUM mice exhibited anomalies in regions where cardiac valve tissue interdigitates with adjacent muscle for support. Ectopic connective and\/or myocardial tissue(s) was associated with the more severe cardiac valve anomalies in FMOD; LUM deficient mice. At postnatal day 0 (P0) there was an increase in the mesenchymal cell number in the regions where valve cusps anchor in FMOD; LUM deficient mice compared to WT. The cardiac valve anomalies correlated with the highest levels of FMOD expression in the heart and also where myotendinous junctions (MTJ) components biglycan, collagen type I alpha 1, and collagen type VI, are also localized. <jats:styled-content><jats:bold>Conclusions<\/jats:bold>:<\/jats:styled-content> The postnatal assembly of the collagen\u2010rich ECM in regions where cardiac valves anchor, that we have designated \u2018myotendinous\u2010like junctions\u2019 (MTLJ) requires the SLRPs FMOD and LUM. Moreover, FMOD and LUM may facilitate mesenchymal cell differentiation in late stages of cardiac valve development. <jats:italic>Developmental Dynamics 245:1029\u20131042, 2016<\/jats:italic>. \u00a9 2016 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24435","type":"journal-article","created":{"date-parts":[[2016,8,9]],"date-time":"2016-08-09T04:47:51Z","timestamp":1470718071000},"page":"1029-1042","source":"Crossref","is-referenced-by-count":15,"title":["Development of myotendinous\u2010like junctions that anchor cardiac valves requires fibromodulin and lumican"],"prefix":"10.1002","volume":"245","author":[{"given":"Loren E.","family":"Dupuis","sequence":"first","affiliation":[{"name":"Department of Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston South Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorna","family":"Doucette","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston South Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. Kittrell","family":"Rice","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston South Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashton E.","family":"Lancaster","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston South Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew G.","family":"Berger","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston South Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shukti","family":"Chakravarti","sequence":"additional","affiliation":[{"name":"Department of Medicine Johns Hopkins University School of Medicine Baltimore Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine B.","family":"Kern","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine and Cell Biology Medical University of South Carolina Charleston South Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwf065"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2008.07.004"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M602919200"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-0804"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-5956-12-25"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20868"},{"issue":"2","key":"e_1_2_6_8_1","first-page":"53","article-title":"The development of the myotendinous junction","volume":"2","author":"Charvet B","year":"2012","journal-title":"A review. 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