{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:15:24Z","timestamp":1787318124986,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,9,18]],"date-time":"2016-09-18T00:00:00Z","timestamp":1474156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["U01HL0981"],"award-info":[{"award-number":["U01HL0981"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>\n                      14\u20103\u20103\u03b5 plays an important role in the maturation of the compact ventricular myocardium by modulating the cardiomyocyte cell cycle via p27\n                      <jats:sup>kip1<\/jats:sup>\n                      . However, additional cardiac defects are possible given the ubiquitous expression pattern of this protein.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Germ line deletion of 14\u20103\u20103\u03b5 led to malalignment of both the outflow tract (OFT) and atrioventricular (AV) cushions, with resulting tricuspid stenosis and atresia, mitral valve abnormalities, and perimembranous ventricular septal defects (VSDs). We confirmed myocardial non\u2010compaction and detected a spongy septum with muscular VSDs and blebbing of the epicardium. These defects were associated with abnormal patterning of p27\n                      <jats:sup>kip1<\/jats:sup>\n                      expression in the subendocardial and possibly the epicardial cell populations. In addition to abnormal pharyngeal arch artery patterning, we found deep endocardial recesses and paucity of intramyocardial coronary vasculature as a result of defective coronary plexus remodeling.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      The malalignment of both endocardial cushions provides a new explanation for tricuspid and mitral valve defects, while myocardial non\u2010compaction provides the basis for the abnormal coronary vasculature patterning. These abnormalities might arise from p27\n                      <jats:sup>kip1<\/jats:sup>\n                      dysregulation and a resulting defect in epithelial\u2010to\u2010mesenchymal transformation. These data suggest that 14\u20103\u20103\u03b5, in addition to left ventricular non\u2010compaction (LVNC), might be linked to different forms of congenital heart disease (CHD).\n                      <jats:italic>Developmental Dynamics 245:1107\u20131123, 2016<\/jats:italic>\n                      . \u00a9 2016 Wiley Periodicals, Inc.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/dvdy.24440","type":"journal-article","created":{"date-parts":[[2016,8,31]],"date-time":"2016-08-31T19:56:54Z","timestamp":1472673414000},"page":"1107-1123","source":"Crossref","is-referenced-by-count":16,"title":["14\u20103\u20103epsilon controls multiple developmental processes in the mouse heart"],"prefix":"10.1002","volume":"245","author":[{"given":"Adriana C.","family":"Gittenberger\u2010de Groot","sequence":"first","affiliation":[{"name":"Department of Cardiology Leiden University Medical Center Leiden The Netherlands"},{"name":"Department of Anatomy and Embryology Leiden University Medical Center Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Hoppenbrouwers","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology Leiden University Medical Center Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucile","family":"Miquerol","sequence":"additional","affiliation":[{"name":"Aix\u2010Marseille University, CNRS IBDM Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Kosaka","sequence":"additional","affiliation":[{"name":"Department of Pediatrics (Neonatology) University of Utah School of Medicine Salt Lake City Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert E.","family":"Poelmann","sequence":"additional","affiliation":[{"name":"Biology Institute Leiden University Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lambertus J.","family":"Wisse","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Embryology Leiden University Medical Center Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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