{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:58:04Z","timestamp":1760403484258,"version":"build-2065373602"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,10,4]],"date-time":"2017-10-04T00:00:00Z","timestamp":1507075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006833","name":"University of South Carolina Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006833","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01HL126747"],"award-info":[{"award-number":["R01HL126747"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"NEI","doi-asserted-by":"publisher","award":["1R01EY024581"],"award-info":[{"award-number":["1R01EY024581"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:p><jats:styled-content><jats:bold>Background:<\/jats:bold><\/jats:styled-content> The formation of healthy heart valves throughout embryonic development is dependent on both genetic and epigenetic factors. Hemodynamic stimuli are important epigenetic regulators of valvulogenesis, but the resultant molecular pathways that control valve development are poorly understood. Here we describe how the heart and valves recover from the removal of a partial constriction (banding) of the OFT\/ventricle junction (OVJ) that temporarily alters blood flow velocity through the embryonic chicken heart (HH stage 16\/17). Recovery is described in terms of 24\u2010 and 48\u2010hr gene expression, morphology, and OVJ hemodynamics. <jats:bold><jats:styled-content>Results:<\/jats:styled-content><\/jats:bold> Collectively, these studies show that after 24\u2009hr of recovery, important epithelial\u2010mesenchymal transformation (EMT) genes TGF\u00dfRIII and Cadherin 11 (CDH11) transcript levels normalize return to control levels, in contrast to Periostin and TGF\u00df,3 which remain altered. In addition, after 48\u2009hr of recovery, TGF\u00df3 and CDH11 transcript levels remain normalized, whereas TGF\u00dfRIII and Periostin are down\u2010regulated. Analyses of OFT cushion volumes in the hearts show significant changes, as does the ratio of cushion to cell volume at 24\u2009hr post band removal (PBR). Morphologically, the hearts show visible alteration following band removal when compared to their control age\u2010matched counterparts. <jats:bold><jats:styled-content>Conclusions:<\/jats:styled-content><\/jats:bold> Although some aspects of the genetic\/cellular profiles affected by altered hemodynamics seem to be reversed, not all gene expression and cardiac growth normalize following 48\u2009hr of band removal. <jats:italic>Developmental Dynamics 247:531\u2013541, 2018<\/jats:italic>. \u00a9 2017 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24588","type":"journal-article","created":{"date-parts":[[2017,9,7]],"date-time":"2017-09-07T21:22:55Z","timestamp":1504819375000},"page":"531-541","source":"Crossref","is-referenced-by-count":10,"title":["Removing vessel constriction on the embryonic heart results in changes in valve gene expression, morphology, and hemodynamics"],"prefix":"10.1002","volume":"247","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4932-9643","authenticated-orcid":false,"given":"Vinal","family":"Menon","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Anatomy University of South Carolina School of Medicine Columbia South Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F.","family":"Eberth","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Anatomy University of South Carolina School of Medicine Columbia South Carolina"},{"name":"Biomedical Engineering Program, College of Engineering and Computing University of South Carolina Columbia South Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorain","family":"Junor","sequence":"additional","affiliation":[{"name":"Instrumentation Resource Facility University of South Carolina School of Medicine Columbia South 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