{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:51:37Z","timestamp":1790790697513,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,4,23]],"date-time":"2018-04-23T00:00:00Z","timestamp":1524441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AA020753"],"award-info":[{"award-number":["R01AA020753"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P50AA011999"],"award-info":[{"award-number":["P50AA011999"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AA024840"],"award-info":[{"award-number":["R21AA024840"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:p><jats:styled-content><jats:bold>Background:<\/jats:bold><\/jats:styled-content> Hepatic stellate cells (HSCs) play an important role in liver fibrogenesis. However, little is known about their phenotype and role in liver development. The aim of this study is to identify specific markers for embryonic HSCs. <jats:styled-content><jats:bold>Results:<\/jats:bold><\/jats:styled-content> Using antibodies against ALCAM and PDPN, we separated mesothelial cells (MCs) and HSCs from developing livers and identified integrin \u03b18 (ITGA8) as a marker for embryonic desmin+ HSCs that are preferentially localized near the developing liver surface and \u03b1\u2010smooth muscle actin+ perivascular mesenchymal cells around the vein. A cell lineage\u2013tracing study revealed that upon differentiation, MC\u2010derived HSCs or perivascular mesenchymal cells express ITGA8 during liver development. Using anti\u2010ITGA8 antibodies, we succeeded in isolating MC\u2010derived HSCs and perivascular mesenchymal cells from embryonic livers. In direct co\u2010culture, ITGA8+ mesenchymal cells promoted the expression of hepatocyte and cholangiocyte markers in hepatoblasts. In the normal adult liver, expression of ITGA8 was restricted to portal fibroblasts in the portal triad. Upon liver injury, myofibroblasts increased the expression of ITGA8. <jats:styled-content><jats:bold>Conclusions:<\/jats:bold><\/jats:styled-content> ITGA8 is a specific cell surface marker of MC\u2010derived HSCs and perivascular mesenchymal cells in the developing liver. Our data suggest that ITGA8+ mesenchymal cells maintain the phenotype of hepatoblast in liver development. <jats:italic>Developmental Dynamics 247:867\u2013881, 2018<\/jats:italic>. \u00a9 2018 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24634","type":"journal-article","created":{"date-parts":[[2018,4,17]],"date-time":"2018-04-17T13:25:11Z","timestamp":1523971511000},"page":"867-881","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Isolation of a unique hepatic stellate cell population expressing integrin \u03b18 from embryonic mouse livers"],"prefix":"10.1002","volume":"247","author":[{"given":"Tomohiro","family":"Ogawa","sequence":"first","affiliation":[{"name":"Southern California Research Center for ALPD and Cirrhosis and Department of Pathology Keck School of Medicine of the University of Southern California Los Angeles California"},{"name":"Center for the Advancement of Higher Education, Faculty 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