{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T12:50:24Z","timestamp":1789563024762,"version":"build-2803163510"},"reference-count":56,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,9,9]],"date-time":"2018-09-09T00:00:00Z","timestamp":1536451200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:p>\n<jats:styled-content>Background:<\/jats:styled-content> The nitric oxide synthase interacting protein (Nosip) has been associated with diverse human diseases including psychological disorders. In line, early neurogenesis of mouse and <jats:italic>Xenopus<\/jats:italic> is impaired upon Nosip deficiency. Nosip knockout mice show craniofacial defects and the down\u2010regulation of Nosip in the mouse and <jats:italic>Xenopus<\/jats:italic> leads to microcephaly. Until now, the exact underlying molecular mechanisms of these malformations were still unknown. <jats:styled-content>Results:<\/jats:styled-content> Here, we show that <jats:italic>nosip<\/jats:italic> is expressed in the developing ocular system as well as the anterior neural crest cells of <jats:italic>Xenopus laevis<\/jats:italic>. Furthermore, Nosip inhibition causes severe defects in eye formation in the mouse and <jats:italic>Xenopus<\/jats:italic>. Retinal lamination as well as dorso\u2013ventral patterning of the retina were affected in Nosip\u2010depleted <jats:italic>Xenopus<\/jats:italic> embryos. Marker gene analysis using <jats:italic>rax, pax6<\/jats:italic> and <jats:italic>otx2<\/jats:italic> reveals an interference with the eye field induction and differentiation. A closer look on Nosip\u2010deficient <jats:italic>Xenopus<\/jats:italic> embryos furthermore reveals disrupted cranial cartilage structures and an inhibition of anterior neural crest cell induction and migration shown by <jats:italic>twist, snai2<\/jats:italic>, and <jats:italic>egr2<\/jats:italic>. Moreover, <jats:italic>foxc1<\/jats:italic> as downstream factor of retinoic acid signalling is affected upon Nosip deficiency. <jats:styled-content>Conclusions:<\/jats:styled-content> Nosip is a crucial factor for the development of anterior neural tissue such the eyes and neural crest cells. <jats:italic>Developmental Dynamics 247:1070\u20101082, 2018<\/jats:italic>. \u00a9 2018 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24659","type":"journal-article","created":{"date-parts":[[2018,7,29]],"date-time":"2018-07-29T03:28:32Z","timestamp":1532834912000},"page":"1070-1082","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Nosip functions during vertebrate eye and cranial cartilage development"],"prefix":"10.1002","volume":"247","author":[{"given":"Hannah","family":"Flach","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Molecular Biology Ulm University  Ulm 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