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These cells give rise to the parasympathetic cardiac ganglia, the smooth muscle layer of the great vessels, some cardiomyocytes, and the conotruncal cushions and aorticopulmonary septum of the outflow tract. Ablation of cardiac neural crest cells results in defective patterning of each of these structures. Previous studies have shown that targeted deletion of the <jats:italic>forkhead transcription factor C2 (Foxc2)<\/jats:italic>, results in cardiac phenotypes similar to that derived from cardiac neural crest cell ablation. <jats:styled-content>Results<\/jats:styled-content>: We report that <jats:italic>Foxc2<\/jats:italic><jats:sup><jats:italic>\u2010\/\u2010<\/jats:italic><\/jats:sup> embryos on the 129s6\/SvEv inbred genetic background display persistent truncus arteriosus and hypoplastic ventricles before embryonic lethality. <jats:italic>Foxc2<\/jats:italic> loss\u2010of\u2010function resulted in perturbed cardiac neural crest cell migration and their reduced contribution to the outflow tract as evidenced by lineage tracing analyses together with perturbed expression of the neural crest cell markers <jats:italic>Sox10<\/jats:italic> and <jats:italic>Crabp1. Foxc2<\/jats:italic> loss\u2010of\u2010function also resulted in alterations in <jats:italic>PlexinD1, Twist1<\/jats:italic>, PECAM1, and <jats:italic>Hand1\/2<\/jats:italic> expression in association with vascular and ventricular defects. <jats:styled-content>Conclusions<\/jats:styled-content>: Our data indicate <jats:italic>Foxc2<\/jats:italic> is required for proper migration of cardiac neural crest cells, septation of the outflow tract, and development of the ventricles. <jats:italic>Developmental Dynamics<\/jats:italic> 247:1286\u20131296, 2018. \u00a9 2018 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/dvdy.24684","type":"journal-article","created":{"date-parts":[[2018,10,30]],"date-time":"2018-10-30T18:03:58Z","timestamp":1540922638000},"page":"1286-1296","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["<i>Foxc2<\/i> is required for proper cardiac neural crest cell migration, outflow tract septation, and ventricle expansion"],"prefix":"10.1002","volume":"247","author":[{"given":"Kimberly E.","family":"Inman","sequence":"first","affiliation":[{"name":"Department of Natural Sciences Shawnee State University  Portsmouth Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo Donato","family":"Caiaffa","sequence":"additional","affiliation":[{"name":"Stowers Institute for Medical Research  Kansas City Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin R.","family":"Melton","sequence":"additional","affiliation":[{"name":"Section of Neonatology, Pulmonary and Perinatal Biology, Cincinnati Children's Hospital  Cincinnati Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1735-8223","authenticated-orcid":false,"given":"Lisa L.","family":"Sandell","sequence":"additional","affiliation":[{"name":"Department of Oral Immunology &amp; Infectious Diseases, School of Dentistry University of Louisville  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