{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T02:35:30Z","timestamp":1787884530834,"version":"build-2784847793"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T00:00:00Z","timestamp":1603670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R35 HL150713"],"award-info":[{"award-number":["R35 HL150713"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01 AR076342"],"award-info":[{"award-number":["R01 AR076342"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Vitamin D is an essential nutrient that has long been known to regulate skeletal growth and integrity. In models of major appendage regeneration, treatment with vitamin D analogs has been reported to improve aspects of zebrafish fin regeneration in specific disease or gene misexpression contexts, but also to disrupt pattern in regenerating salamander limbs. Recently, we reported strong mitogenic roles for vitamin D signaling in several zebrafish tissues throughout life stages, including epidermal cells and osteoblasts of adult fins. To our knowledge, molecular genetic approaches to dissect vitamin D function in appendage regeneration have not been described.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Using a knock\u2010in GFP reporter for the expression of the vitamin D target gene and negative regulator <jats:italic>cyp24a1<\/jats:italic>, we identified active vitamin D signaling in adult zebrafish fins during tissue homeostasis and regeneration. Transgenic expression of <jats:italic>cyp24a1<\/jats:italic> or a dominant\u2010negative vitamin D receptor (VDR) inhibited regeneration of amputated fins, whereas global vitamin D treatment accelerated regeneration. Using tissue regeneration enhancer elements, we found that local enhancement of VDR expression could improve regeneration with low doses of a vitamin D analog.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Vitamin D signaling enhances the efficacy of fin regeneration in zebrafish.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.261","type":"journal-article","created":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T12:37:03Z","timestamp":1602851823000},"page":"1330-1339","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Regulation of zebrafish fin regeneration by vitamin D signaling"],"prefix":"10.1002","volume":"250","author":[{"given":"Anzhi","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Cell Biology Duke University Medical Center  Durham North Carolina USA"},{"name":"Regeneration Next Duke University  Durham North Carolina 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