{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:11:41Z","timestamp":1790403101055,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T00:00:00Z","timestamp":1611532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Male infertility is a prevalent clinical presentation for which there is likely a strong genetic component due to the thousands of genes required for spermatogenesis. Within this study we investigated the role of the gene <jats:italic>Scrn1<\/jats:italic> in male fertility. <jats:italic>Scrn1<\/jats:italic> is preferentially expressed in XY gonads during the period of sex determination and in adult Sertoli cells based on single cell RNA sequencing. We investigated the expression of <jats:italic>Scrn1<\/jats:italic> in juvenile and adult tissues and generated a knockout mouse model to test its role in male fertility.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Scrn1<\/jats:italic> was expressed at all ages examined in the post\u2010natal testis; however, its expression peaked at postnatal days 7\u201014 and SCRN1 protein was clearly localized to Sertoli cells. <jats:italic>Scrn1<\/jats:italic> deletion was achieved via removal of exon 3, and its loss had no effect on male fertility or sex determination. Knockout mice were capable of siring litters of equal size to wild type counterparts and generated equal numbers of sperm with comparable motility and morphology characteristics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>Scrn1<\/jats:italic> was found to be dispensable for male fertility, but this study identifies SCRN1 as a novel marker of the Sertoli cell cytoplasm.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.299","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T13:11:04Z","timestamp":1610716264000},"page":"922-931","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["The Sertoli cell expressed gene secernin\u20101 (<i>Scrn1<\/i>) is dispensable for male fertility in the mouse"],"prefix":"10.1002","volume":"250","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1078-756X","authenticated-orcid":false,"given":"Brendan J.","family":"Houston","sequence":"first","affiliation":[{"name":"School of Biological Sciences Monash University  Clayton Victoria Australia"},{"name":"School of BioSciences The University of Melbourne  Parkville Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liina","family":"Nagirnaja","sequence":"additional","affiliation":[{"name":"Division of Genetics Oregon National Primate Research Center  Beaverton Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Jo","family":"Merriner","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Monash University  Clayton Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne E.","family":"O'Connor","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Monash University  Clayton Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidenobu","family":"Okuda","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Monash University  Clayton Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenan","family":"Omurtag","sequence":"additional","affiliation":[{"name":"Division of Reproductive Endocrinology and Infertility School of Medicine, Washington University  St Louis Missouri USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Smith","sequence":"additional","affiliation":[{"name":"Biomedicine Discovery Institute Monash University  Clayton Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth I.","family":"Aston","sequence":"additional","affiliation":[{"name":"Department of Surgery (Urology) University of Utah  Salt Lake City Utah USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald F.","family":"Conrad","sequence":"additional","affiliation":[{"name":"Division of Genetics Oregon National Primate Research Center  Beaverton Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7298-4940","authenticated-orcid":false,"given":"Moira K.","family":"O'Bryan","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Monash University  Clayton Victoria Australia"},{"name":"School of BioSciences The University of Melbourne  Parkville Victoria 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