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Here, we generated <jats:italic>Eya2<\/jats:italic> knockout (<jats:italic>Eya2<\/jats:italic><jats:sup><jats:italic>\u2212<\/jats:italic><\/jats:sup>) and a <jats:italic>lacZ<\/jats:italic> knockin reporter (<jats:italic>Eya2<\/jats:italic><jats:sup><jats:italic>lacZ<\/jats:italic><\/jats:sup>) mice and performed a detailed expression analysis for <jats:italic>Eya2<\/jats:italic><jats:sup><jats:italic>lacZ<\/jats:italic><\/jats:sup> at different developmental stages to trace <jats:italic>Eya2<\/jats:italic><jats:sup><jats:italic>lacZ<\/jats:italic><\/jats:sup>\u2010positive cells in <jats:italic>Eya2<\/jats:italic>\u2010null mice. We describe that <jats:italic>Eya2<\/jats:italic> is not only expressed in cranial sensory and dorsal root ganglia, retina and olfactory epithelium, and somites as previously reported, but also <jats:italic>Eya2<\/jats:italic> is specifically detected in other organs during mouse development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that <jats:italic>Eya2<\/jats:italic> is expressed in ocular and trochlear motor neurons. In the inner ear, <jats:italic>Eya2<\/jats:italic><jats:sup><jats:italic>lacZ<\/jats:italic><\/jats:sup> is specifically expressed in differentiating hair cells in both vestibular and cochlear sensory epithelia of the inner ear and <jats:italic>Eya2<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> or <jats:italic>Eya2<\/jats:italic><jats:sup><jats:italic>lacZ\/lacZ<\/jats:italic><\/jats:sup> mice displayed mild hearing loss. Furthermore, we detected <jats:italic>Eya2<\/jats:italic> expression during both salivary gland and thymus development and <jats:italic>Eya2<\/jats:italic>\u2010null mice had a smaller thymus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>As <jats:italic>Eya2<\/jats:italic> is coexpressed with other members of the Eya family genes, these results together highlight that <jats:italic>Eya2<\/jats:italic> as a potential regulator may act synergistically with other Eya genes to regulate the differentiation of the inner ear sensory hair cells and the formation of the salivary gland and thymus.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.326","type":"journal-article","created":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T06:45:53Z","timestamp":1615704353000},"page":"1450-1462","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["<scp><i>Eya2<\/i><\/scp> expression during mouse embryonic development revealed by <scp><i>Eya2<\/i><sup><i>lacZ<\/i><\/sup><\/scp> knockin reporter and homozygous mice show mild hearing loss"],"prefix":"10.1002","volume":"250","author":[{"given":"Ting","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Genetics and Genomic Sciences Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinshu","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genomic Sciences Icahn School of Medicine at Mount Sinai  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8957-6780","authenticated-orcid":false,"given":"Pin\u2010Xian","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genomic Sciences Icahn School of Medicine at Mount Sinai  New York New York USA"},{"name":"Department of Cell, Developmental and Regenerative 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