{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T06:23:15Z","timestamp":1790662995180,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T00:00:00Z","timestamp":1617148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81400479"],"award-info":[{"award-number":["81400479"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870738"],"award-info":[{"award-number":["81870738"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670954"],"award-info":[{"award-number":["81670954"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Mutation in Odontogenesis\u2010associated phosphoprotein (ODAPH) has been reported to cause recessive hypomineralized amelogenesis imperfecta (AI) in human. However, the exact role of ODAPH in amelogenesis is still unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ODAPH was identified as a novel constituent of the atypical basal lamina located at the interface between maturation ameloblasts and the enamel by dual immunofluorescence staining of ODAPH and LAMC2. <jats:italic>Odaph<\/jats:italic> knockout mice were generated to explore the function of ODAPH in amelogenesis. <jats:italic>Odaph<\/jats:italic><jats:sup><jats:italic>\u2212\/\u2212<\/jats:italic><\/jats:sup> mice teeth showed severely attrition and reduced enamel mineralization. Histological analysis showed from transition or early\u2010maturation stage, ameloblasts were rapidly shortened, lost cell polarity, and exhibited cell pathology. Abundant enamel matrix marked by amelogenin was retained. Temporary cyst\u2010like structures were formed between flattened epithelial cells and the enamel from maturation stage to eruption. The integrity of the atypical basal lamina was impaired indicated by the reduced diffuse expression of LAMC2 and AMTN. The expression of maturation stage related genes of <jats:italic>Amtn<\/jats:italic>, <jats:italic>Klk4<\/jats:italic>, <jats:italic>Integrin\u03b26<\/jats:italic> and <jats:italic>Slc24a4<\/jats:italic> were significantly decreased.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results suggested <jats:italic>Odaph<\/jats:italic> played vital roles during amelogenesis by maintaining the integrity of the atypical basal lamina in maturation stage, which may contribute to a better understanding of the pathophysiology of human AI.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.336","type":"journal-article","created":{"date-parts":[[2021,3,27]],"date-time":"2021-03-27T00:40:30Z","timestamp":1616805630000},"page":"1505-1517","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Maturation stage enamel defects in Odontogenesis\u2010associated phosphoprotein (<i>Odaph<\/i>) deficient mice"],"prefix":"10.1002","volume":"250","author":[{"given":"Yikang","family":"Ji","sequence":"first","affiliation":[{"name":"Department of Stomatology Yantai Affiliated Hospital of Binzhou Medical University  Yantai Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Preventive Dentistry Hospital Affiliated to Binzhou Medical University  Binzhou Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Preventive Dentistry Hospital Affiliated to Binzhou Medical University  Binzhou Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Preventive Dentistry Hospital Affiliated to Binzhou Medical University  Binzhou Shandong 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