{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T07:43:22Z","timestamp":1773906202056,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,7,17]],"date-time":"2021-07-17T00:00:00Z","timestamp":1626480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["254046"],"award-info":[{"award-number":["254046"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The Transforming Growth Factor \u03b2 (TGF\u03b2) family is a group of related proteins that signal through a type I and type II receptors. Betaglycan, also known as the type III receptor (Tgfbr3), is a coreceptor for various ligands of the TGF\u03b2 family that participates in heart, liver and kidney development as revealed by the <jats:italic>tgfbr3<\/jats:italic>\u2010null mouse, as well as in angiogenesis as revealed by Tgfbr3 downregulation in morphant zebrafish.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Here, we present CRISPR\/Cas9\u2010derived zebrafish Tgfbr3\u2010null mutants, which exhibited unaltered embryonic angiogenesis and developed into fertile adults. One reproducible phenotype displayed by these <jats:italic>Tgfbr3<\/jats:italic>\u2010null mutants is delayed chordacentra mineralization, which nonetheless does not result in vertebral abnormalities in the adult fishes. We also report that the canonical TGF\u03b2 signaling pathway is needed for proper chordacentra mineralization and that Tgfbr3 absence decreases this signal in the notochordal cells responsible for this process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Betaglycan's \u201cligand presentation\u201d function contributes to the optimal TGF\u03b2 signaling required for zebrafish chordacentra mineralization.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.393","type":"journal-article","created":{"date-parts":[[2021,7,6]],"date-time":"2021-07-06T09:50:25Z","timestamp":1625565025000},"page":"213-225","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Chordacentrum mineralization is delayed in zebrafish betaglycan\u2010null mutants"],"prefix":"10.1002","volume":"251","author":[{"given":"Tonatiuh","family":"Molina\u2010Villa","sequence":"first","affiliation":[{"name":"Department of Cellular and Developmental Biology, Institute of Cellular Physiology UNAM  M\u00e9xico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lizbeth","family":"Ram\u00edrez\u2010Vidal","sequence":"additional","affiliation":[{"name":"Department of Cellular and Developmental Biology, Institute of Cellular Physiology UNAM  M\u00e9xico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valent\u00edn","family":"Mendoza","sequence":"additional","affiliation":[{"name":"Department of Cellular and Developmental Biology, Institute of Cellular Physiology UNAM  M\u00e9xico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Escalante\u2010Alcalde","sequence":"additional","affiliation":[{"name":"Division of Neurosciences, Department of Neural Development and Physiology, Institute of Cellular Physiology UNAM  M\u00e9xico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3689-1824","authenticated-orcid":false,"given":"Fernando","family":"L\u00f3pez\u2010Casillas","sequence":"additional","affiliation":[{"name":"Department of Cellular and Developmental Biology, Institute of Cellular Physiology UNAM  M\u00e9xico City 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