{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T07:55:25Z","timestamp":1778658925179,"version":"3.51.4"},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,11,12]],"date-time":"2021-11-12T00:00:00Z","timestamp":1636675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008431","name":"Consejer\u00eda de Educaci\u00f3n, Junta de Castilla y Le\u00f3n","doi-asserted-by":"publisher","award":["CSI143P20"],"award-info":[{"award-number":["CSI143P20"]}],"id":[{"id":"10.13039\/501100008431","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011885","name":"Medical Engineering Centre, King\u2019s College London","doi-asserted-by":"publisher","award":["BFU2004\u201000860\/BFI"],"award-info":[{"award-number":["BFU2004\u201000860\/BFI"]}],"id":[{"id":"10.13039\/100011885","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Fibroblast growth factors (Fgfs) are required for survival and organ formation during embryogenesis. Fgfs often execute their functions redundantly. Previous analysis of <jats:italic>Fgf3<\/jats:italic> mutants revealed effects on inner ear formation and embryonic survival with incomplete penetrance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Here, we show that presence of a <jats:italic>neomycin<\/jats:italic> resistance gene (<jats:italic>neo<\/jats:italic>) replacing the <jats:italic>Fgf3<\/jats:italic> coding region leads to reduced survival during embryogenesis and an increased penetrance of inner ear defects. <jats:italic>Fgf3<\/jats:italic><jats:sup><jats:italic>neo\/neo<\/jats:italic><\/jats:sup> mutants showed reduced expression of <jats:italic>Fgf4<\/jats:italic>, which is positioned in close proximity to the <jats:italic>Fgf3<\/jats:italic> locus in the mouse genome. Conditional inactivation of <jats:italic>Fgf4<\/jats:italic> during inner ear development on a <jats:italic>Fgf3<\/jats:italic> null background using <jats:italic>Fgf3\/4 cis mice<\/jats:italic> revealed a redundant requirement between these <jats:italic>Fgfs<\/jats:italic> during otic placode induction. In contrast, inactivation of <jats:italic>Fgf3<\/jats:italic> and <jats:italic>Fgf4<\/jats:italic> in the pharyngeal region where both <jats:italic>Fgfs<\/jats:italic> are also co\u2010expressed using a <jats:italic>Foxg1<\/jats:italic>\u2010Cre driver did not affect development of the pharyngeal arches. However, these mutants showed reduced perinatal survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results highlight the importance of Fgf signaling during development. In particular, different members of the Fgf family act redundantly to guarantee inner ear formation and embryonic survival.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.435","type":"journal-article","created":{"date-parts":[[2021,10,31]],"date-time":"2021-10-31T22:53:25Z","timestamp":1635720805000},"page":"877-884","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Inactivation of <i>Fgf3<\/i> and <i>Fgf4<\/i> within the <i>Fgf3\/Fgf4\/Fgf15<\/i> gene cluster reveals their redundant requirement for mouse inner ear induction and embryonic survival"],"prefix":"10.1002","volume":"251","author":[{"given":"Laura","family":"Zelarayan","sequence":"first","affiliation":[{"name":"Institute of Pharmacology and Toxicology University Medical Center Goettingen (UMG)  Goettingen Germany"},{"name":"DZHK (German Center for Cardiovascular Research) partner site Goettingen  Germany"},{"name":"Cluster of Excellence \u201cMultiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells\u201d (MBExC) University of Goettingen  Germany"},{"name":"Unidad de Excelencia, Instituto de Biolog\u00eda y Gen\u00e9tica Molecular Universidad de Valladolid y Consejo Superior de Investigaciones Cient\u00edficas  Valladolid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Vendrell","sequence":"additional","affiliation":[{"name":"Unidad de Excelencia, Instituto de Biolog\u00eda y Gen\u00e9tica Molecular Universidad de Valladolid y Consejo Superior de Investigaciones Cient\u00edficas  Valladolid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Dom\u00ednguez\u2010Frutos","sequence":"additional","affiliation":[{"name":"Unidad de Excelencia, Instituto de Biolog\u00eda y Gen\u00e9tica Molecular Universidad de Valladolid y Consejo Superior de Investigaciones Cient\u00edficas  Valladolid 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