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Some vertebrates, such as newts and zebrafish, regenerate the heart throughout their lives. To understand how regenerative abilities differ among animal species, comparative research has been conducted in animals like mouse, zebrafish, and newt. For those purposes, cryo\u2010injury is suitable as an experimental model for the pathological condition of human myocardial infarction. In fact, cryo\u2010injury procedures are common in mouse and zebrafish.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the present study, we induced cryo\u2010damage on the ventricle in Iberian ribbed newts using a liquid nitrogen\u2010chilled probe. We observed that the injured area recovered within 8\u2009weeks, with remodeling of scar tissue and proliferation of cardiomyocytes. We investigated the subsequent recovery of cryo\u2010injured and amputated tissues by comparative analysis of the gene expression profiles following these two procedures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Notably, we established a cryo\u2010injury procedure for the newt and confirmed that regeneration of the cryo\u2010damaged myocardial tissue is achieved by changes in gene expression that are milder than those observed in the amputation model. Our results suggest that the cryo\u2010injury method is suitable for comparing the process of cardiac regeneration in the newt with that in other animal models.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.450","type":"journal-article","created":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T20:45:50Z","timestamp":1640724350000},"page":"864-876","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Cryo\u2010injury procedure\u2010induced cardiac regeneration shows unique gene expression profiles in the newt <i>Pleurodeles waltl<\/i>"],"prefix":"10.1002","volume":"251","author":[{"given":"Hitoshi","family":"Uemasu","sequence":"first","affiliation":[{"name":"Division of Pediatrics and Perinatology, School of Medicine, Faculty of Medicine Tottori University  Yonago Tottori 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