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Since genetic perturbations and disease states may have localized effects, data from individual cranial bones are valuable for the interpretation of experimental assays. We employ high\u2010resolution microcomputed tomography to characterize cranial bones of C57BL\/6J mice at embryonic day (E) 15.5 and E17.5, day of birth (P0), and postnatal day 7 (P7) and provide estimates of individual bone volume and tissue mineral density (TMD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Average volume and TMD values are reported for individual bones. Significant differences in volume and TMD during embryonic ages likely reflect early mineralization of cranial neural crest\u2010derived and intramembranously forming bones. Although bones of the face and vault had higher TMD values during embryonic ages, bones of the braincase floor had significantly higher TMD values by P7.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These ontogenetic data on cranial bone volume and TMD serve as a reference standard for future studies using mice bred on a C57BL\/6J genetic background. Our findings also highlight the importance of differentiating \u201ccontrol\u201d data from mice that are presented as \u201cunaffected\u201d littermates, particularly when carrying a single copy of a cre\u2010recombinase gene.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.458","type":"journal-article","created":{"date-parts":[[2022,1,29]],"date-time":"2022-01-29T03:14:25Z","timestamp":1643426065000},"page":"1196-1208","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Embryonic and early postnatal cranial bone volume and tissue mineral density values for <scp>C57BL<\/scp>\/<scp>6J<\/scp> laboratory mice"],"prefix":"10.1002","volume":"251","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9537-5750","authenticated-orcid":false,"given":"Kate M.","family":"Lesciotto","sequence":"first","affiliation":[{"name":"College of Osteopathic Medicine Sam Houston State University  Conroe Texas 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