{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T04:19:59Z","timestamp":1780373999641,"version":"3.54.1"},"reference-count":36,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,4,23]],"date-time":"2022-04-23T00:00:00Z","timestamp":1650672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20H03264"],"award-info":[{"award-number":["20H03264"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["anatomypubs.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Developmental Dynamics"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Axolotls can regenerate their limbs. In their limb regeneration process, developmental genes are re\u2010expressed and reorganize the developmental axes, in which the position\u2010specific genes are properly re\u2010expressed. However, how such position specificity is reorganized in the regeneration processes has not been clarified. To address this issue, we focused on the reactivation process of <jats:italic>Lmx1b<\/jats:italic>, which determines the limb dorsal identity in many animals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Here, we show that <jats:italic>Lmx1b<\/jats:italic> expression is maintained in the dorsal skin before amputation and is activated after amputation. Furthermore, we demonstrate that only cells located in the dorsal side prior to limb amputation could reactivate <jats:italic>Lmx1b<\/jats:italic> after limb amputation. We also found that <jats:italic>Lmx1b<\/jats:italic> activation was achieved by nerve presence. The nerve factors, BMP2+FGF2+FGF8 (B2FF), consistently reactivate <jats:italic>Lmx1b<\/jats:italic> when applied to the dorsal skin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These results imply that the retained <jats:italic>Lmx1b<\/jats:italic> expression in the intact skin plays a role in positional memory, which instruct cells about the spatial positioning before amputation. This memory is reactivated by nerves or nerve factors that can trigger the entire limb regeneration process. Our findings highlight the role of nerves in amphibian limb regeneration, including both the initiation of limb regeneration and the reactivation of position\u2010specific gene expression.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.476","type":"journal-article","created":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T04:08:43Z","timestamp":1649650123000},"page":"1509-1523","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<i>Lmx1b<\/i> activation in axolotl limb regeneration"],"prefix":"10.1002","volume":"251","author":[{"given":"Sakiya","family":"Yamamoto","sequence":"first","affiliation":[{"name":"Faculty of Science, Department of Biological Sciences Okayama University  Okayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rena","family":"Kashimoto","sequence":"additional","affiliation":[{"name":"Graduate School of Natural Science and Technology Okayama University  Okayama 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