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Here, we examine the role of mllt3 in developmental hematopoiesis during embryogenesis using zebrafish.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cloning, sequencing, phylogenetic, and synteny analyses showed high evolutionary conservation between important functional domains of the zebrafish orthologue of mllt3 and MLLT3 in humans. Quantitative reverse transcription\u2010PCR and in situ hybridization analyses revealed that <jats:italic>mllt3<\/jats:italic> is maternally supplied and zygotically expressed throughout embryonic development, and that expression is highest between 10 and 24\u2009hours post\u2010fertilization (hpf) coincident with enrichment in the intermediate cell mass (ICM) and posterior blood island, which are the sites of the primitive and transient definitive hematopoiesis in zebrafish, respectively. Further, we found co\u2010expression of <jats:italic>mllt3<\/jats:italic> with the early hematopoietic progenitor markers <jats:italic>tal1<\/jats:italic>, <jats:italic>gata2<\/jats:italic>, and <jats:italic>gata1a<\/jats:italic> in the posterior ICM. By investigating zebrafish hematopoietic mutants, we discovered that mllt3 is involved in erythroid precursor formation. By 48\u201072\u2009hpf, <jats:italic>mllt3<\/jats:italic> expression proved to be restricted to non\u2010hematopoietic tissues including head structures, pronephric tubules, and liver primordium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings establish a link between mllt3 and primitive erythropoiesis and provide the basis for future functional investigations.<\/jats:p><\/jats:sec>","DOI":"10.1002\/dvdy.477","type":"journal-article","created":{"date-parts":[[2022,4,16]],"date-time":"2022-04-16T07:14:30Z","timestamp":1650093270000},"page":"1728-1740","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Leukemia\u2010associated transcription factor <i>mllt3<\/i> is important for primitive erythroid development in zebrafish embryogenesis"],"prefix":"10.1002","volume":"251","author":[{"given":"Giuseppe","family":"Germano","sequence":"first","affiliation":[{"name":"Division of Hematology\/Oncology Institute of Pediatric Research Citt\u00e0 Della Speranza  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Porazzi","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology, Sidney Kimmel Cancer Center Thomas Jefferson University  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn A.","family":"Felix","sequence":"additional","affiliation":[{"name":"Division of Oncology, Department of Pediatrics The Children's Hospital of Philadelphia  Philadelphia Pennsylvania 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